In this work, we aim to develop cancer cell‐targeting AIE dots based on a polyyne‐bridged red‐emissive AIEgen, 2TPE‐4E, through the combination of metabolic engineering and bio‐orthogonal reactions. Azide groups on a tumor were efficiently produced by intravenous injection of Ac4ManNAz and glycol‐metabolic engineering. These bio‐orthogonal azide groups could facilitate the specific targeting of DBCO‐AIE dots to the tumor cells undergoing metal‐free click reaction in vivo. The efficiency of this targeting strategy could be further improved with the development of new bio‐orthogonal chemical groups with higher reactivity and a large amount of AIEgens could be delivered to the tumor for diagnosis.

中文翻译：

---

基于Polyyne-bridged红色发射AIEgen的生物正交AIE点用于肿瘤代谢标记和靶向成像

在这项工作中，我们旨在通过代谢工程和生物正交反应相结合，开发基于多炔桥式红色发射AIEgen，2TPE-4E的靶向癌细胞的AIE点。通过静脉内注射Ac4ManNAz和乙二醇代谢工程可以有效地产生肿瘤上的叠氮化物基团。这些生物正交叠氮基团可以促进DBCO‐AIE点对体内发生无金属点击反应的肿瘤细胞的特异性靶向。通过开发具有更高反应性的新的生物正交化学基团，可以进一步提高这种靶向策略的效率，并且可以将大量的AIEgens传递给肿瘤进行诊断。