Adult hippocampal neurogenesis (AHN) is implicated in brain adaptations and disease pathogenesis. A seminal study showed adult-born neurons in the subgranular zone (SGZ) of the dentate gyrus (DG) in cancer patients 58–72 years of age, detecting bromodeoxyuridine co-localization with neuronal markers (Eriksson et al., 1998). Subsequently, human AHN was reported using immunohistochemistry targeting markers expressed by neuronal cells at different maturational stages,in situhybridization (ISH), and14C decay-defined neuronal age (Spalding et al., 2013Spalding K.L.Bergmann O.Alkass K.Bernard S.Salehpour M.Huttner H.B.Boström E.Westerlund I.Vial C.Buchholz B.A.et al.Dynamics of hippocampal neurogenesis in adult humans.Cell.2013;153:1219-1227AbstractFull TextFull Text PDFPubMedScopus (690)Google Scholar). Detection of AHN markers is dependent on methodological approaches such as brain tissue processing and fixation, postmortem interval, and other factors affecting tissue antigenicity and preservation of proteins and mRNAs (Kempermann et al., 2018Kempermann G.Gage F.H.Aigner L.Song H.Curtis M.A.Thuret S.Kuhn H.G.Jessberger S.Frankland P.W.Cameron H.A.et al.Human adult neurogenesis: evidence and remaining questions.Cell Stem Cell.2018;23:25-30AbstractFull TextFull Text PDFScopus (0)Google Scholar). This discussion intends to clarify the divergent findings between our group (Boldrini et al., 2018Boldrini M.Fulmore C.A.Tartt A.N.Simeon L.R.Pavlova I.Poposka V.Rosoklija G.B.Stankov A.Arango V.Dwork A.J.et al.Human hippocampal neurogenesis persists throughout aging.Cell Stem Cell.2018;22:589-599.e5AbstractFull TextFull Text PDFPubMedScopus (49)Google Scholar) and the Alvarez-Buylla group (Sorrells et al., 2018Sorrells S.F.Paredes M.F.Cebrian-Silla A.Sandoval K.Qi D.Kelley K.W.James D.Mayer S.Chang J.Auguste K.I.et al.Human hippocampal neurogenesis drops sharply in children to undetectable levels in adults.Nature.2018;555:377-381CrossrefPubMedScopus (79)Google Scholar,Paredes et al., 2018Paredes M.F.Sorrells S.F.Cebrian-Silla A.Sandoval K.Qi D.Kelley K.W.James D.Mayer S.Chang J.Auguste K.I.et al.Does adult neurogenesis persist in the human hippocampus?.Cell Stem Cell.2018;23(this issue):780-781Google Scholar) and elucidate key factors surrounding conflicting conclusions. Human Hippocampal Neurogenesis Persists throughout AgingBoldrini et al.Cell Stem CellApril 05, 2018In BriefFull-TextPDFHuman Adult Neurogenesis: Evidence and Remaining QuestionsKempermann et al.Cell Stem CellApril 19, 2018In BriefIn BriefFull-TextPDFDoes Adult Neurogenesis Persist in the Human Hippocampus?Paredes et al.Cell Stem CellDecember 06, 2018In BriefIn BriefFull-TextPDF

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评估成人和衰老人脑海马神经发生程度的注意事项

成人海马神经发生 (AHN) 与大脑适应和疾病发病机制有关。一项开创性研究表明，在 58-72 岁的癌症患者的齿状回 (DG) 的颗粒下区 (SGZ) 中，成年出生的神经元检测到溴脱氧尿苷与神经元标记物的共定位 (Eriksson 等人，1998)。随后，报告了人类 AHN 使用免疫组织化学靶向标记物，该标记物由处于不同成熟阶段、原位杂交 (ISH) 和 14 C 衰变定义的神经元年龄的神经元细胞表达（Spalding 等人，2013 Spalding KLBergmann O.Alkass K.Bernard S.Salehpour M. Huttner HBBoström E.Westerlund I.Vial C.Buchholz BAet al.Dynamics of hippocampal neurogenesis in adult humans.Cell.2013;153:1219-1227AbstractFull TextFull Text PDFPubMedScopus (690)Google Scholar)。AHN 标记物的检测取决于方法学方法，例如脑组织处理和固定、死后间隔以及影响组织抗原性和蛋白质和 mRNA 保存的其他因素（Kempermann 等人，2018Kempermann G.Gage FHAigner L.Song H.Curtis MAThuret S.Kuhn HGJessberger S.Frankland PWCameron HAet al.Human adult neurogenesis: evidence and remaining questions.Cell Stem Cell.2018;23:25-30AbstractFull TextFull Text PDFScopus (0)Google Scholar)。本次讨论旨在澄清我们小组之间的不同发现（Boldrini 等人，2018Boldrini M.Fulmore CATartt ANSimeon LRPavlova I.Poposka V.Rosoklija GBStankov A.Arango V.Dwork AJet al.Human hippocampal neurogenesis persists throughout aging.Cell Stem Cell .2018;22:589-599。e5AbstractFull TextFull Text PDFPubMedScopus (49)Google Scholar) and the Alvarez-Buylla group (Sorrells et al., 2018Sorrells SFParedes MFCebrian-Silla A.Sandoval K.Qi D.Kelley KWJames D.Mayer S.Chang J.Auguste KIet al.Human儿童海马神经发生急剧下降到成人无法检测到的水平。Nature.2018;555:377-381CrossrefPubMedScopus (79)Google Scholar,Paredes et al., 2018Paredes MFSorrells SFCebrian-Silla A.Sandoval K.Qi D.Kelley KWJames D.Mayer S.Chang J.Auguste KIet al.Does adult neurogenesis persist in the human hippocampus?.Cell Stem Cell.2018;23(this issue):780-781Google Scholar) 并阐明围绕相互矛盾结论的关键因素。人类海马神经发生在整个 AgingBoldrini 等人中持续存在。细胞干细胞 2018 年 4 月 5 日简述全文 PDF 人类成人神经发生：