In this work, a restricted access volatile supramolecular solvent (RAM-VOL-SUPRAS) directly synthesized in milk is proposed for the first time for the simultaneous extraction and cleanup of benzimidazole anthelmintic drugs in milk meant for human consumption. The RAM-VOL-SUPRAS was formed by the self-assembly and coacervation of hexanol in tetrahydrofuran induced by the water content in milk. Benzimidazoles legislated by the European Union were quantitatively extracted (80–110%), and proteins were precipitated by the action of THF and the amphiphile; extraction of carbohydrates was avoided by a size exclusion mechanism, and lipids were removed during hexanol evaporation. The analytical methodology was fully validated according to Commission Decision 2002/657/EC. Method detection limits from 0.03 to 0.14 μg L^–1 were well below the maximum residue limits legislated in milk for these drugs, with interday precisions at maximum residue levels below 13%. This novel methodology guarantees a rapid and reliable tool for daily and routine laboratory analyses in the field of food quality control.

中文翻译：

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LC-MS / MS之前用于牛奶中苯并咪唑驱虫药的一步提取/净化的限制进入的挥发性超分子溶剂

在这项工作中，首次提出了在牛奶中直接合成的限制进入的挥发性超分子溶剂（RAM-VOL-SUPRAS），用于同时萃取和净化供人食用的牛奶中的苯并咪唑驱虫药。RAM-VOL-SUPRAS是由牛奶中水分引起的四氢呋喃中己醇的自组装和凝聚作用形成的。定量提取了欧盟立法规定的苯并咪唑（80-110％），并且在四氢呋喃和两亲物的作用下沉淀了蛋白质。通过尺寸排阻机制避免了碳水化合物的提取，并且在己醇蒸发过程中去除了脂质。该分析方法已根据委员会决定2002/657 / EC进行了充分验证。方法检测限从0.03到0.14μgL ^–1均远低于这些药物在牛奶中规定的最大残留限量，在最大残留量低于13％时的日间精确度。这种新颖的方法为食品质量控制领域的日常和常规实验室分析提供了一种快速可靠的工具。