Bioorthogonal transformation of prodrugs and profluorophores using transition metal catalysts (TMCs) offers a promising strategy for therapeutic and imaging applications. Here, we report the surface engineering of nanoparticles to specifically localize gold nanoparticles (AuNPs) with encapsulated TMCs (nanozymes) to either the inside or outside of cells. The ability to control nanozyme localization and hence activity was demonstrated by the activation of pro-fluorophores and prodrugs intra- and extracellularly, establishing the potential of engineered nanozyme platforms for both diagnostic and therapeutic purposes.

中文翻译：

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使用表面工程纳米酶控制细胞内和细胞外生物正交催化。

使用过渡金属催化剂（TMC）对前药和前荧光团进行生物正交转化为治疗和成像应用提供了一种有前途的策略。在这里，我们报告了纳米颗粒的表面工程，以专门将金纳米颗粒（AuNPs）与封装的TMC（纳米酶）定位到细胞的内部或外部。通过在细胞内和细胞外激活前荧光团和前药，证明了控制纳米酶定位和活性的能力，从而建立了用于诊断和治疗目的的工程化纳米酶平台的潜力。