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Mineralized layered films of xanthan and chitosan stabilized by polysaccharide interactions: A promising material for bone tissue repair
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2018-12-05 , DOI: 10.1016/j.carbpol.2018.12.006
Aline E. Aguiar , Mariana de O. Silva , Andrea C.D. Rodas , Celso A. Bertran

Mineralized films are interesting biomaterials to repair bone defects. They can be easily shaped into bone defect and be permeated by body fluids as well as allow cell proliferation. Xanthan and chitosan films mineralized with hydroxyapatite prepared and characterized in this work showed an improved film stability and controlled swelling degree when dipped in different pH buffers. The layer-by-layer technique used in the film's preparation associated with the behaviour at different pH allowed to explore separately each interaction – polysaccharide-polysaccharide and polysaccharide-ions. The entanglement between polysaccharides, the interaction of the oppositely charged polysaccharides ionic groups (amide for chitosan and carboxylate for xanthan) and the interaction with Ca2+ ions confers a pH-responsive behaviour to the films. The mineralization with in situ hydroxyapatite formation resulted in an additional stability in the mineral phase. It has lower crystallinity similar to bone mineral as confirmed by X-Ray diffractogram. The films that were dipped in calcium phosphate solution during their production had positive results with in vitro cell adhesion test using MG63 cells culture.



中文翻译:

多糖相互作用稳定的黄原胶和壳聚糖矿化层状膜:有望修复骨组织的材料

矿化膜是修复骨缺损的有趣生物材料。它们很容易被塑造成骨缺损,并被体液渗透并允许细胞增殖。制备并表征的由羟基磷灰石矿化的黄原胶和脱乙酰壳多糖薄膜在不同的pH缓冲液中浸渍时,具有改善的薄膜稳定性和溶胀度。薄膜制备中使用的逐层技术与在不同pH值下的行为有关,从而可以分别探讨每种相互作用-多糖-多糖和多糖-离子。多糖之间的缠结,带相反电荷的多糖离子基团的相互作用(壳聚糖为酰胺,黄原胶为羧酸盐)以及与Ca 2+的相互作用离子赋予膜以pH响应的行为。具有原位羟基磷灰石形成的矿化作用在矿相中产生了额外的稳定性。X射线衍射图证实,它具有较低的结晶度,类似于骨矿物质。在生产过程中将其浸在磷酸钙溶液中的膜在使用MG63细胞培养的体外细胞粘附试验中获得了积极的结果。

更新日期:2018-12-05
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