The present work describes design and synthesis of dicyanovinylpyrene as a molecular probe 1. The photophysical studies of probe 1 shows intramolecular charge transfer (ICT) behavior. Probe 1 upon interaction with different amino acids in partial aqueous medium shows high selectivity for cysteine (Cys) through a chemical reaction based chemodosimeter approach. The observed change in the photophysical behavior and fluorescence “turn–on” response upon interaction with Cys is attributed to the formation of Michael type adduct in the medium. The mode of interaction between probe 1 and Cys has been confirmed by the ¹HNMR, ¹³CNMR, HRMS spectroscopic data and significant change in the morphological feature.

中文翻译：

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基于迈克尔反应的简单“开启”荧光化学计量仪，用于检测部分水性介质中的半胱氨酸

本工作描述了作为分子探针1的双氰基乙烯基in的设计与合成。探针1的光物理研究显示了分子内电荷转移（ICT）行为。探针1与部分氨基酸在部分水性介质中的相互作用通过基于化学反应的化学计量法显示出对半胱氨酸（Cys）的高选择性。与Cys相互作用后，观察到的光物理行为和荧光“开启”响应的变化归因于介质中迈克尔型加合物的形成。探针1与Cys之间的相互作用模式已通过¹ HNMR ¹³确认。CNMR，HRMS光谱数据和形态特征发生重大变化。