Iron is an essential element for many physiological functions of several organisms but in excess it causes toxicity. High iron content in water bodies of mountainous states is considered as one of the major factor, responsible for low productivity in aquaculture systems. But, till date comprehensive reports on the adverse effect of iron overload in aquatic organisms, especially cultured fishes are scanty. Therefore, the present study was undertaken to investigate the adverse effects of iron overload in economically important aquaculture fish species Labeo rohita. Three sub-lethal test concentration of iron (ferrous) viz., 1/16th, 1/8th and 1/4th of LC₅₀ (post 96 h) i.e. 8.25, 16.51 and 33.01 mg L⁻¹, respectively, were used for in vivo exposure. Blood cells and tissue samples of the control & exposed specimens were sampled at intervals of 24, 48, 72 and 96 h to assess alterations in hematological, oxidative stress and histological parameters. Significant changes in erythrocyte and leukocyte counts, hemoglobin, lipid peroxidation, antioxidant enzyme activity (super oxide dismutase and catalase) and tissue iron accumulation were observed in the exposed fish. Significant increase in lipid peroxidation, coupled with significant reduction in free radicals scavengers like super oxide dismutase and catalase revealed a compromised anti-oxidative defense mechanism in the fishes exposed to iron overload. Histological examination of gills and liver showed severe tissue injury and histological alternations. Severity was found to increase in time and concentration dependent manner. Perl's staining revealed accumulation of excess iron in liver of the exposed fish. The observed patho-physiological changes in the present study provide the most comprehensive insight of iron overload stress in L. rohita.

铁是几种生物体许多生理功能所必需的元素，但过量会引起毒性。山区水体中的高铁含量被认为是造成水产养殖系统生产力低下的主要因素之一。但是，迄今为止，关于铁超载对水生生物特别是养殖鱼类的不利影响的综合报道很少。因此，本研究旨在研究铁超载对经济上重要的水产养殖鱼类Labeo rohita的不利影响。铁（亚铁）的三个亚致命测试浓度，即LC ₅₀（96小时后）的1 / 16、1 / 8和1/4，即8.25、16.51和33.01 mg L ^-1分别用于体内暴露。对照和暴露标本的血细胞和组织样本每隔24、48、72和96小时取样一次，以评估血液学，氧化应激和组织学参数的变化。在暴露的鱼中观察到红细胞和白细胞计数，血红蛋白，脂质过氧化，抗氧化酶活性（超氧化物歧化酶和过氧化氢酶）和组织铁积累的显着变化。脂质过氧化作用的显着增加，以及自由基清除剂（如超氧化物歧化酶和过氧化氢酶）的显着减少，揭示了暴露于铁超负荷的鱼的抗氧化防御机制受损。腮和肝脏的组织学检查显示严重的组织损伤和组织学改变。发现严重性以时间和浓度依赖性方式增加。Perl的染色表明，暴露的鱼肝中有过多的铁蓄积。本研究中观察到的病理生理变化提供了L中铁超负荷应激的最全面的见解。罗希塔。