A new turn‐on fluorescent probe 2‐((pyren‐3‐yl) methyl) isoindoline‐1,3‐dione was synthesized in a single step, high yielding reaction to detect hydrazine in solution. This ligand is highly sensitive to detect hydrazine in micromolar (1.1 μM) concentration in solution. It has high selectivity towards hydrazine compared to other analytes. Different biophysical experiments like hydrazine screening from both tap water and distilled water, vapour phase detection, anti‐interference studies with different analytes also corroborate the phenomenon of selective detection of hydrazine by our ligand. NMR titration and mass spectrum established the mechanism of hydrazine sensing. In addition, the probe was successfully used to trap exogenously added hydrazine inside the cell, which was further utilized to study insitu hydrazine released from an anti‐tubercular drug Isoniazid (INH), after its metabolism.

中文翻译：

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通过新型开启式荧光探针对作为异烟肼代谢物的肼进行细胞检测：合成，活细胞成像和体外毒性研究

一个新的开启式荧光探针2-（（pyren-3-yl）甲基）isoindoline-1,3-dione一步合成了高产率的反应，可检测溶液中的肼。该配体对检测溶液中微摩尔（1.1μM）浓度的肼非常敏感。与其他分析物相比，它对肼具有高选择性。不同的生物物理实验，例如从自来水和蒸馏水中进行肼筛选，气相检测，对不同分析物的抗干扰研究，也证实了配体对肼的选择性检测现象。NMR滴定和质谱建立了肼感测的机理。此外，该探针已成功用于捕获细胞内外源添加的肼，