Abstract In this study, Deep Eutectic Solvents (DESs) were utilized for ultrasound-assisted emulsification liquid phase microextraction (UA-ELPME) of Patent Blue V in syrup and water samples. Patent Blue V in sample solution of pH 4.0 was extracted into DES phase prepared from choline chloride and phenol. Concentration of Patent Blue V in final DES phase was measured at 627.5 nm with Uv-Vis spectrophotometry. Sample volume, pH, matrix effect, volume and type of DES, ultrasonication and centrifugation times were investigated and optimized. The developed UA-ELPME method presents a limit of detection (LOD) of 0.37 μg L−1, preconcentration factor of 15 and a relative standard deviation (RSD, %) of 6.0% under the optimum conditions. The validation was carried out with standard addition-recovery experiments for syrup and water samples. The obtained recovery results showed that the developed UA-ELPME method was applicable for routine Patent Blue V analysis in syrup and water samples.

中文翻译：

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开发一种基于深共熔溶剂的新型简单超声辅助乳化液相微萃取方法，用于糖浆和水样中的专利蓝 V

摘要 本研究采用深共晶溶剂 (DESs) 对糖浆和水样中的专利蓝 V 进行超声辅助乳化液相微萃取 (UA-ELPME)。将pH 4.0的样品溶液中的专利蓝V萃取到由氯化胆碱和苯酚制备的DES相中。使用紫外-可见分光光度法在 627.5 nm 处测量最终 DES 相中专利蓝 V 的浓度。对样品体积、pH、基质效应、DES 的体积和类型、超声处理和离心时间进行了研究和优化。在最佳条件下，开发的 UA-ELPME 方法的检测限 (LOD) 为 0.37 μg L-1，预浓缩因子为 15，相对标准偏差 (RSD，%) 为 6.0%。通过糖浆和水样的标准添加-回收实验进行验证。