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A simple and convenient method for the hydrolysis of styrene-maleic anhydride copolymers to styrene-maleic acid copolymers
Chemistry and Physics of Lipids ( IF 3.4 ) Pub Date : 2018-12-01 , DOI: 10.1016/j.chemphyslip.2018.11.011
Adrian H. Kopf , Martijn C. Koorengevel , Cornelis A. van Walree , Timothy R. Dafforn , J. Antoinette Killian

Styrene-maleic acid (SMA) copolymers are increasingly gaining attention in the membrane protein field due to their ability to solubilize lipid membranes into discoidal nanoparticles. The copolymers are synthesized as styrene-maleic anhydride (SMAnh), and need to be converted to the free acid form (SMA) before they are capable of solubilizing membranes. This hydrolysis reaction is traditionally performed under rather cumbersome reflux conditions. Here we report an alternative method for the hydrolysis reaction using simple and readily available equipment found in virtually all biochemical laboratories, namely an autoclave. Based on the results we propose an optimum set of standard conditions for the hydrolysis reaction, that should make the method easily accessible to a wide scope of researchers.



中文翻译:

一种简便的将苯乙烯-马来酸酐共聚物水解为苯乙烯-马来酸共聚物的方法

苯乙烯-马来酸(SMA)共聚物由于能够将脂质膜溶解成盘状纳米颗粒,因此在膜蛋白领域越来越受到关注。共聚物以苯乙烯-马来酸酐(SMAnh)的形式合成,因此必须先转化为游离酸形式(SMA)才能溶解膜。传统上,该水解反应在相当繁琐的回流条件下进行。在这里,我们报告了使用几乎所有生化实验室中都可以找到的简单易用的设备(即高压釜)进行水解反应的另一种方法。根据结果​​,我们提出了一套最佳的水解反应标准条件,这将使该方法易于广泛的研究人员使用。

更新日期:2018-12-01
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