The structural characteristics of the polysaccharides from Aralia elata root barks (AERP) were systematically investigated by FT-IR, HPSEC-ELSD and colorimetric methods as well as by GC-MS based monosaccharide compositions, Smith degradations, and methylation analysis. The result showed average molecular weights of AERP were between 42.7 kDa and 93.9 kDa. AERP was composed of Ara, Rha, GlcA, Man, Glc, and Gal in a molar ratio of 22.2: 10.3: 8.1: 32.7: 5.7: 21.2 along with a small number of sulfate (3.38%) and acetyl (4.87%) groups. The abundant glycosidic linkages of Man, Ara, Gal, and Rha were observed as more than 90% of all the monosaccharides detected. Studies to evaluate hepatoprotective potentials of AERP showed that they had potent hepatoprotective effects in vivo in carbon tetrachloride-induced acute liver injury (CIALI) in mice by histopathological evaluation, biochemical examinations and ELISA assays. GC-MS was further used to determine the effects of AERP on the chemical profiles of nine common short-chain fatty acids (SCFAs) produced by intestinal flora metabolism in CIALI mice. These findings not only provide novel insights into the pharmacological actions of AERP on the protection from CIALI in mice, but they also demonstrate that determining SCFA profiles by targeted GC-MS metabolomics is an effective technique to investigate the molecular mechanisms of the effects of plant polysaccharides on intestinal flora metabolism.

通过FT-IR，HPSEC-ELSD和比色法以及基于GC-MS的单糖组成，Smith降解和甲基化分析，系统地研究了Aralia elata根皮（AERP）多糖的结构特征。结果显示AERP的平均分子量在42.7 kDa和93.9 kDa之间。AERP由Ara，Rha，GlcA，Man，Glc和Gal组成，摩尔比为22.2：10.3：8.1：32.7：5.7：21.2，以及少量硫酸盐（3.38％）和乙酰基（4.87％） 。观察到Man，Ara，Gal和Rha的丰富糖苷键占检测到的所有单糖的90％以上。评估AERP的保肝潜力的研究表明，它们在体内具有强大的保肝作用通过组织病理学评估，生化检查和ELISA测定法检测四氯化碳诱导的小鼠急性肝损伤（CIALI）。GC-MS还用于确定AERP对CIALI小鼠肠道菌群代谢产生的9种常见短链脂肪酸（SCFA）的化学谱的影响。这些发现不仅提供了对AERP预防小鼠CIALI的药理作用的新颖见解，而且还证明了通过靶向GC-MS代谢组学确定SCFA谱是研究植物多糖作用的分子机制的有效技术。对肠道菌群的新陈代谢。