Development of new replacement grafts for anterior cruciate ligament (ACL) repair requires mechanical testing to ensure they can provide joint stability following implantation. A decellularised porcine superflexor tendon (pSFT) has been developed previously as an alternative to current reconstruction methods and subjected to biomechanical analysis. The application of varied strain rates to biological tissues is known to alter their biomechanical properties, however the effects of decellularisation on strain rate dependent and dynamic mechanical behaviour of tissues have not been explored. This study utilised tensile testing to investigate the material properties of native and decellularised pSFTs at three different strain rates (1%.s⁻¹, 10%.s⁻¹ and 100%.s⁻¹). In addition, dynamic mechanical analysis (DMA) was used to ascertain the relative contributions of the solid and fluid phase components of the tissues.

Ultimate tensile strength was significantly reduced in decellularised compared with native untreated pSFTs but was unaffected by strain rate. In contrast, toe region moduli increased with increasing strain rate for native tissues, but this effect was not observed in decellularised pSFTs. Linear region moduli were unaffected by strain rate, but were significantly reduced in decellularised pSFT compared with native tissue.

Following DMA, significant reductions in dynamic modulus, storage modulus and loss modulus were seen in decellularised compared with native pSFT. Interestingly, the damping ability of the tendons was unaffected by decellularisation, suggesting that solid and fluid phases of the tissue were affected equally. These results, alongside previous studies, suggest that decellularisation affects collagen crimp, tissue swelling and collagen fibre sliding. However, despite these findings, the biomechanical properties of decellularised pSFT remain sufficient to act as an off-the-shelf solution for ACL reconstruction.

开发用于前十字韧带（ACL）修复的新替代移植物需要进行机械测试，以确保它们在植入后能够提供关节稳定性。脱细胞猪超屈肌腱（pSFT）先前已开发作为当前重建方法的替代方法，并经过了生物力学分析。已知将变化的应变速率应用于生物组织会改变它们的生物力学特性，但是尚未研究脱细胞作用对组织的应变速率依赖性和动态机械行为的影响。本研究中使用拉伸试验在三个不同的应变率（1％.S调查天然和脱细胞pSFTs的材料特性^-1，10％的.s ^-1和100％.S ^-1）。此外，动态力学分析（DMA）用于确定组织的固相和液相成分的相对贡献。

与天然未处理的pSFT相比，脱细胞后的极限拉伸强度显着降低，但不受应变速率的影响。相反，对于天然组织，脚趾区域的模量随应变率的增加而增加，但是在脱细胞的pSFTs中未观察到这种作用。线性区域模量不受应变率的影响，但与天然组织相比，脱细胞的pSFT显着降低。

DMA后，与天然pSFT相比，脱细胞后的动态模量，储能模量和损耗模量显着降低。有趣的是，肌腱的阻尼能力不受脱细胞作用的影响，表明组织的固相和液相均受到相同的影响。这些结果以及以前的研究表明，脱细胞作用会影响胶原蛋白的卷曲，组织肿胀和胶原蛋白纤维的滑动。然而，尽管有这些发现，脱细胞的pSFT的生物力学性能仍然足以充当ACL重建的现成解决方案。