In this study, ultrasound-assisted extraction (UAE) of phenolic compounds in baobab (Adansonia digitata) seeds was optimised using RSM. Based on the single factor experiment results, the effect of four parameters, extraction time (10 - 20 min), temperature (40 - 60°C), % amplitude (30 - 50) and solvent to solid ratio (20-30, mL/g) on Total Flavonoids Content (TFC) were investigated and optimised using a central composite design (CCD). The predicted optimal conditions with the highest desirably (0.874) were: 20 min, 30% amplitude, 60°C temperature and 30 mL/g solvent to solid ratio. Under these conditions, the experimental TFC value (1633.84 ± 10.75 mg RE/100 g DW) was highly correlated with the predicted value (1631.77 mg RE/100 g DW). Moreover, a comparative study confirmed that the recovery of TFC, TPC and antioxidant activity (DPPH and FRAP) was significantly higher in UAE than maceration and Heat Assisted Extraction (HAE) extraction methods in addition to a shorter extraction time. HPLC analysis of the UAE optimised sample extract revealed the presence of 10 phenolic compounds with the highest concentration of D-(+) catechin (87.18 ± 14.57 µg/g). This study is the first attempt to optimise UAE parameters for the extraction of phenolic compounds in baobab seeds.

在这项研究中，超声辅助提取在猴面包酚类化合物（UAE）（猴面包树种子使用RSM进行了优化。基于单因素实验结果，四个参数的影响分别为：萃取时间（10-20分钟），温度（40-60°C），振幅％（30-50）和溶剂与固体的比例（20-30，mL） / g）总黄酮含量（TFC）进行了研究，并使用了中央复合设计（CCD）进行了优化。具有最高期望值（0.874）的预测最佳条件是：20分钟，30％振幅，60°C温度和30 mL / g溶剂与固体的比率。在这些条件下，实验TFC值（1633.84±10.75 mg RE / 100 g DW）与预测值（1631.77 mg RE / 100 g DW）高度相关。此外，一项比较研究证实，TFC的回收率，阿联酋的TPC和抗氧化剂活性（DPPH和FRAP）比浸渍和热辅助萃取（HAE）萃取方法要高得多，而且萃取时间更短。对阿联酋优化的样品提取物进行的HPLC分析表明，存在10种酚类化合物，它们的D-（+）儿茶素浓度最高（87.18±14.57 µg / g）。这项研究是为提取猴面包树种子中的酚类化合物而优化阿联酋参数的首次尝试。