Alkaline phosphatase (ALP) is associated with many diseases, and its accurate detection is of great significance. Fluorescent compounds with aggregation‐induced emission (AIE) feature show beneficial advantages for serving as fluorescent probes. Herein, an AIE‐active “turn on” probe for ALP detection was synthesized through incorporating a strong electron‐withdrawing group (cyano) in the middle and the recognition moiety phosphate group at the end, thereby rendering a D–A–D structure with a relatively high conjugation degree and good water solubility. It was found that the probe TPE‐CN‐pho is highly sensitive to ALP in aqueous solution. In the presence of ALP, the hydrophilic phosphate group on the probe is rapidly removed, resulting in a decrease in water solubility and subsequent formation of aggregates, thereby achieving aggregation‐induced emission. Moreover, the probe TPE‐CN‐pho has also been successfully applied to imaging ALP in living cells.

中文翻译：

---

具有聚集诱导发射的荧光探针，用于检测碱性磷酸酶和细胞成像

碱性磷酸酶（ALP）与多种疾病有关，其准确检测具有重要意义。具有聚集诱导发射（AIE）功能的荧光化合物显示出用作荧光探针的有益优势。在此，通过在中间引入一个强吸电子基团（氰基）并在末端引入一个识别部分磷酸基团，合成了一种用于ALP检测的具有AIE活性的“开启”探针，从而使D–A–D结构具有较高的结合度和良好的水溶性。发现TPE-CN-pho探针对水溶液中的ALP高度敏感。在存在ALP的情况下，探针上的亲水性磷酸基团会迅速被去除，从而导致水溶性降低并随后形成聚集体，从而实现聚集引发的排放。此外，探针TPE-CN-pho也已成功应用于活细胞中的ALP成像。