Hornification are usually occurred in cellulosic fibers and even in nanocellulose fibrils during dehydration process but their mesoscopic structural changes is not fully understood. Here we investigated the structural features of cellulose nanocrystals (CNCs) and cellulose nanofibrils (CNF) with different solid content. In order to avoid the effect of drying, liquid AFM mode was carried out in this work. The different water contents of nanocellulose were quantified by Differential Scanning Calorimetry (DSC). We showed evidence of extreme growth in radial direction in the redispersed nanocelluloses, analyzed by statistics of contours from microscopy images. The amount of free water, freezing bound water, and non-freezing bound water decreased gradually during water evaporation and were completely removed in sequence. In addition, the hydroxyl accessibility of both redispersed CNCs and CNF were reduced by 83.33% and 81.96% via evaluating the uptake of fluorescent dyes.

在脱水过程中，角质化通常发生在纤维素纤维中，甚至发生在纳米纤维素原纤维中，但它们的介观结构变化尚不完全清楚。在这里，我们研究了具有不同固体含量的纤维素纳米晶体（CNC）和纤维素纳米原纤维（CNF）的结构特征。为了避免干燥的影响，在这项工作中进行了液体AFM模式。通过差示扫描量热法（DSC）对纳米纤维素的不同水分含量进行定量。我们显示了重新分散的纳米纤维素在径向上极端增长的证据，通过显微镜图像的轮廓统计进行了分析。在水蒸发过程中，游离水，冷冻结合水和非冷冻结合水的量逐渐减少，并依次被完全去除。此外，