A series of cellulosic substrates has been produced, treated with lytic polysaccharide monooxygenase (LPMO) from Streptomyces ambofaciens (SamLPMO10C), and analyzed by high performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD). The activity of the bacterial LPMO showed high variability depending on the origin and degree of crystallinity of the substrate. Additionally, we tested the effectiveness of SamLPMO10C in the nanofibrillation of flax, a high crystalline agricultural fiber, as a single pretreatment or in combination with cellulases. All pretreatments were followed by a mechanical defibrillation by high-pressure homogenization (HPH) to obtain cellulose nanofibrils (NFC). The combined LPMO-cellulase treatment showed higher fibrillation yield, optical transmittance and carboxylate content than control reactions. Therefore, it could be explored as a promising green alternative to reduce the energy consumption in the production of NFC. To our knowledge, this is the first study reporting the effect of a bacterial LPMO in nanocellulose production.

已经生产了一系列纤维素底物，用来自Streptomyces ambofaciens的溶解性多糖单加氧酶（LPMO）处理（SamLPMO10C），并通过带有脉冲安培检测（PAD）的高效阴离子交换色谱（HPAEC）进行分析。细菌LPMO的活性显示出高的可变性，这取决于底物的来源和结晶度。此外，我们测试了SamLPMO10C在亚麻（一种高结晶性农业纤维）的纳米原纤化中作为单一预处理或与纤维素酶联合使用的有效性。所有预处理之后，通过高压均质化（HPH）进行机械除颤，以获得纤维素纳米原纤维（NFC）。组合的LPMO-纤维素酶处理显示出比对照反应更高的原纤化产率，透光率和羧酸盐含量。因此，可以将其探索为减少NFC生产中的能耗的有前途的绿色替代品。