Oligonucleotide-spider silk conjugates can be placed on silicon wafers by complementary DNA strands, which are coupled chemically to the surface. Such specific immobilization of spider silk proteins allows the nucleation and guided growth of β-sheet-rich nanofibrils in the presence of phosphate ions on the surface. Adjustment of the concentration of the immobilized conjugate, phosphate concentration and time of the assembly reaction enables control over fibril surface density and length. Furthermore, soft lithography was used to direct the conjugates on predetermined spots with a submicron resolution yielding high contrast surface patterns. This approach, which combines bottom-up and top-down surface structuring, opens up new possibilities in protein fibril based bionanotechnology.

中文翻译：

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通过DNA定向的蜘蛛丝组件对表面进行纳米级图案化。

寡核苷酸-蜘蛛丝共轭物可以通过互补的DNA链放置在硅片上，互补的DNA链化学偶联到表面上。蜘蛛丝蛋白的这种特异性固定使得在表面上存在磷酸根离子的情况下，富含β-折叠的纳米原纤维的形核和引导生长。固定化缀合物浓度，磷酸盐浓度和组装反应时间的调节使得能够控制原纤维表面密度和长度。此外，使用软光刻法以亚微米分辨率将缀合物引导到预定的斑点上，从而产生高对比度的表面图案。这种结合了自下而上和自上而下的表面结构的方法，为基于蛋白质原纤维的生物纳米技术开辟了新的可能性。