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Effects of Mild Oxidative and Structural Modifications Induced by Argon Plasma on Physicochemical Properties of Actomyosin from King Prawn (Litopenaeus vannamei)
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2018-11-19 00:00:00 , DOI: 10.1021/acs.jafc.8b05178
Flora-Glad Chizoba Ekezie 1 , Jun-Hu Cheng 1 , Da-Wen Sun 1, 2
Affiliation  

In the present work, the structure and physicochemical properties of natural actomyosin (NAM) extracted from king prawn (Litopenaeus vannamei) and subjected to atmospheric pressure plasma jet (APPJ) generated in argon gas as a function of the treatment time were examined. The results revealed that prawn NAM exhibited a correlating decrease in pH from 7.06 ± 0.03 to 6.92 ± 0.02 and a slight increase (p > 0.05) in solubility from 91.89 ± 1.57 to 96.86 ± 1.19 within the first few minutes of plasma exposure as a result of the formation of soluble aggregates. A rise in turbidity was also noted, confirming the occurrence of protein aggregation. These changes were also accompanied by a rise in emulsifying activity from 48.96 ± 1.66 to 67.31 ± 1.39 m2/g (p < 0.05) and a nearly 50% increase in foaming capacity after 5 min of APPJ exposure. The modulation of these properties occurred as a result of conformational changes in NAM evident by various complementary structural analyses conducted. Overall, these findings show that mild oxidation from argon plasma can be used for modification of protein functionality and emphasize the need for optimal selection of plasma processing conditions.

中文翻译:

从大虾肌动球蛋白的理化性质温和氧化和结构修改诱导的影响氩离子(南美白对虾

在目前的工作中,研究了从虾虾(南美对虾)提取的天然肌动球蛋白(NAM)的结构和理化性质,并经过氩气中产生的大气压等离子体射流(APPJ)作为处理时间的函数。结果表明,在等离子暴露的最初几分钟内,虾NAM的pH值从7.06±0.03降低到6.92±0.02,并且溶解度从91.89±1.57略有增加(p > 0.05)到96.86±1.19。可溶性聚集体的形成。还注意到浊度升高,证实了蛋白质聚集的发生。这些变化还伴随着乳化活性从48.96±1.66升至67.31±1.39 m 2 / g(p <0.05),APPJ暴露5分钟后起泡能力提高了近50%。这些特性的调节是NAM构象变化的结果,这种变化可通过进行各种互补的结构分析得出。总体而言,这些发现表明,来自氩等离子体的轻度氧化可用于修饰蛋白质功能,并强调需要对血浆处理条件进行最佳选择。
更新日期:2018-11-19
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