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Light-driven fine chemical production in yeast biohybrids
Science ( IF 56.9 ) Pub Date : 2018-11-15 , DOI: 10.1126/science.aat9777
Junling Guo 1 , Miguel Suástegui 1 , Kelsey K. Sakimoto 2, 3 , Vanessa M. Moody 4 , Gao Xiao 1, 5 , Daniel G. Nocera 2 , Neel S. Joshi 1, 5
Affiliation  

Light-powered cell factories Bacteria and fungi are used industrially to produce commodity fine chemicals at vast scale. Sugars are an economical feedstock, but many of the desired products require enzymatic reduction, meaning that some of the sugar must be diverted to regenerate the cellular reductant NADPH (reduced form of nicotinamide adenine dinucleotide phosphate). Guo et al. show that electrons from light-sensitive nanoparticles can drive reduction of cellular NADPH in yeast, which can then be used for reductive biosynthetic reactions. This system can reduce diversion of carbon to NADPH regeneration and should be compatible with many existing engineered strains of yeast. Science, this issue p. 813 Light-harvesting nanoparticles power reduction reactions in genetically engineered yeast. Inorganic-biological hybrid systems have potential to be sustainable, efficient, and versatile chemical synthesis platforms by integrating the light-harvesting properties of semiconductors with the synthetic potential of biological cells. We have developed a modular bioinorganic hybrid platform that consists of highly efficient light-harvesting indium phosphide nanoparticles and genetically engineered Saccharomyces cerevisiae, a workhorse microorganism in biomanufacturing. The yeast harvests photogenerated electrons from the illuminated nanoparticles and uses them for the cytosolic regeneration of redox cofactors. This process enables the decoupling of biosynthesis and cofactor regeneration, facilitating a carbon- and energy-efficient production of the metabolite shikimic acid, a common precursor for several drugs and fine chemicals. Our work provides a platform for the rational design of biohybrids for efficient biomanufacturing processes with higher complexity and functionality.

中文翻译:

酵母生物杂交体中的光驱动精细化学品生产

光动力细胞工厂 细菌和真菌在工业上用于大规模生产商品精细化学品。糖是一种经济的原料,但许多所需的产品需要酶促还原,这意味着必须转移一些糖以再生细胞还原剂 NADPH(烟酰胺腺嘌呤二核苷酸磷酸的还原形式)。郭等人。表明来自光敏纳米颗粒的电子可以驱动酵母中细胞 NADPH 的还原,然后可用于还原性生物合成反应。该系统可以减少碳向 NADPH 再生的转移,并且应该与许多现有的酵母工程菌株兼容。科学,这个问题 p。813 捕光纳米粒子在基因工程酵母中的功率降低反应。通过将半导体的光捕获特性与生物细胞的合成潜力相结合,无机-生物混合系统具有成为可持续、高效和多功能的化学合成平台的潜力。我们开发了一种模块化生物无机混合平台,该平台由高效的捕光磷化铟纳米颗粒和基因工程酿酒酵母(一种生物制造中的主力微生物)组成。酵母从发光的纳米粒子中收集光生电子,并将它们用于氧化还原辅因子的细胞溶质再生。该过程使生物合成和辅因子再生脱钩,促进代谢物莽草酸的碳和能源高效生产,莽草酸是多种药物和精细化学品的常见前体。
更新日期:2018-11-15
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