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Analysis of glycerophospholipid metabolism after exposure to PCB153 in PC12 cells through targeted lipidomics by UHPLC-MS/MS
Ecotoxicology and Environmental Safety ( IF 6.8 ) Pub Date : 2018-11-13 , DOI: 10.1016/j.ecoenv.2018.11.006
Xinlu Wang , Yanyang Xu , Xiao Song , Qi Jia , Xining Zhang , Yongzhong Qian , Jing Qiu

Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) that have neurotoxicity, reproductive toxicity, hepatotoxicity and immunotoxicity in both animals and humans. Few studies have focused on the changes to endogenous glycerophospholipid metabolism caused by PCB153. To evaluate the relationships between exposure to PCB153 and specific endogenous glycerophospholipid metabolism, an ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was implemented in this study. Twenty-two endogenous glycerophospholipids in PC12 cells were analyzed after exposure to PCB153 at dosages of 0.05 μg mL−1, 0.5 μg mL−1 or 20 μg mL−1 for 120 h. PC(14:0/14:0), PE(16:0/18:1), PE(16:0/18:2), PS(18:0/18:1) and PI(16:0/18:1) were identified as potential biomarkers under the rules of t-test (P) value < 0.05 and variable importance at projection (VIP) value > 1. It was also found that the alterations at 0.05 μg mL−1 and 20 μg mL−1 PCB153 were similar at 120 h, while 0.5 μg mL−1 PCB153 presented an opposite trend. Additionally, significant upregulation of PC, PE and PS with the same fatty acid chains of 18:0/18:2 was found after exposure to 0.05 μg mL−1 and 20 μg mL−1 PCB153 at 120 h. This study revealed that PCB153 exposure modulated 22 endogenous glycerophospholipids in PC12 cells and provided the basis for the further study of PCB153 on the effects of glycerophospholipids on PC12 cells.



中文翻译:

使用UHPLC-MS / MS通过靶向脂质组学分析PC12细胞中PCB153暴露后的甘油磷脂代谢

多氯联苯(PCBs)是持久性有机污染物(POPs),在动物和人类中均具有神经毒性,生殖毒性,肝毒性和免疫毒性。很少有研究关注PCB153引起的内源性甘油磷脂代谢的变化。为了评估PCB153暴露与特定内源性甘油磷脂代谢之间的关系,本研究采用了超高效液相色谱串联质谱法(UHPLC-MS / MS)。二十二个在PC12细胞中内源性甘油曝光后进行分析,以在PCB153 0.05微克毫升剂量-1,0.5微克毫升-1或20微克毫升-1持续120小时。PC(14:0/14:0),PE(16:0/18:1),PE(16:0/18:2),PS(18:0/18:1)和PI(16:0 /根据t检验(P)值<0.05和预测的可变重要性(VIP)值> 1的规则,将18:1)鉴定为潜在的生物标志物。还发现,在0.05μgmL -1和20μg处的变化在120小时时,mL -1 PCB153相似,而0.5μgmL -1 PCB153呈现相反的趋势。另外,暴露于0.05μgmL -1和20μgmL -1后,发现具有18:0/18:2相同脂肪酸链的PC,PE和PS显着上调120小时的PCB153。这项研究表明,PCB153暴露可调节PC12细胞中的22种内源性甘油磷脂,并为进一步研究PCB153甘油磷脂对PC12细胞的作用提供基础。

更新日期:2018-11-13
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