Poor blood flow circulation can occur in the subcortical regions of the brain in many brain diseases. However, the limitations of light penetration imaging techniques hinder the detection of blood flow in deep brain tissues in vivo. Hence, in this Letter, we present a gradient index lens-based laser speckle contrast imaging system for time-lapse blood flow detection in subcortical regions of the brain. We monitored the hemodynamic changes in the thalamus of mouse models of acute hypoxia and transient middle cerebral artery occlusion as a proof of concept for practical applications.

中文翻译：

---

使用显微内窥镜对大脑深部血流进行激光散斑对比成像

在许多脑部疾病中，大脑皮层下区域可能会出现血液循环不良。然而，光穿透成像技术的局限性阻碍了体内深层脑组织中血流的检测。因此，在这封信中，我们提出了一种基于梯度折射率透镜的激光散斑对比度成像系统，用于大脑皮层下区域的延时血流检测。我们监测了急性缺氧和短暂性大脑中动脉闭塞的小鼠模型丘脑的血流动力学变化，作为实际应用概念的证明。