This paper employs a specially designed aggregation‐induced emission fluorogen (AIEgen) to in vivo visualize the process of Hg²⁺ bioaccumulation in a common species of freshwater zooplankton Daphnia carinata (D. carinata) by two methods, direct Hg²⁺ absorption and ingestion of Hg²⁺ contaminated food. We analysed the relevance between photoluminescence (PL, I₅₉₅/I₄₈₀) ratios and Hg²⁺ (C_Hg2+) and developed the master curve for Hg²⁺ determination based on measuring the PL intensity of the solution. Meanwhile, fluorescent image analysis showed that the major recipient organs of Hg²⁺ in D. carinata were the compound eyes and carapace, followed by the intestine and shell gland, but not the brain or heart. The response of D. carinata to Hg²⁺ via uptake from surrounding water differed distinctly from that through food intake of the algae (Euglena gracilis) contaminated by mercury. When Hg²⁺ was encapsulated by algae membrane, no fluorescence was detected, and the carapace morphology remained intact after ingesting algae for 80 min, in contrast to the rapid carapace deformation by direct Hg²⁺ absorption. The D. carinata showed higher mortality by direct Hg²⁺ immersion than via food ingestion. The reason for the high mortality after Hg²⁺ immersion was possibly due to carapace deformity after chemical reaction between chitin and mercury, but the biochemical pathway leading to morality needs further study.

中文翻译：

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新型聚集诱导的氟排放物在蚤蚤中Hg2 +生物富集过程的体内可视化

本文采用专门设计的聚集诱导发射氟（AIEgen）体内可视化两种常见方法中的淡水浮游水蚤水蚤（D. carinata）中Hg ^2+的生物蓄积过程，直接吸收Hg ²⁺和摄入两种方法。被Hg ²⁺污染的食物。我们分析了光致发光（PL，I ₅₉₅ / I ₄₈₀）比与Hg ²⁺（C _{Hg2 +}）之间的相关性，并基于测量溶液的PL强度^绘制了Hg ²⁺测定的主曲线。同时，荧光图像分析显示汞的主要接受器官²⁺在D.芥是复眼和甲壳，其次是肠道和蛋壳腺，而不是大脑或心脏。Carinata藻通过摄入周围水对Hg ²⁺的响应与食物摄入受汞污染的藻类（Euglena gracilis）的响应截然不同。当Hg ²⁺被藻类膜包裹时，未检测到荧光，并且在摄入藻类80分钟后，甲壳形态仍保持完整，这与直接吸收Hg ²⁺引起的甲壳迅速变形相反。该D.芥直接汞具有较高的死亡率²⁺沉浸而不是通过食物摄入。Hg ²⁺浸泡后高死亡率的原因可能是由于甲壳质与汞之间的化学反应后的甲壳变形，但导致道德的生化途径尚需进一步研究。