The impacts of triphenyltin (TPT) on ecological health have been of great concern due to their widespread use and ubiquity in aquatic ecosystems. However, little is known about the effects of TPT on the reproductive behaviors of fishes. Therefore, the present study was conducted to investigate the effects of TPT at environmentally relevant concentrations (0, 1 and 10 ng Sn/L) on the mating behaviors and the attractiveness to females during mating in male guppies (Poecilia reticulata). The results showed that TPT exposure disturbed the mating behaviors; the TPT-exposed male fish performed more sneaking attempts, but no changes in sigmoid courtship were displayed. The increases in sneaking attempts might be related to increases in testosterone levels induced by TPT exposure. In the context of a competing male, the TPT-exposed males were less attractive to females during mating. The decreases in attractiveness might be related to decreases in carotenoid-based coloration, shown as decreases in caudal fin redness values and skin carotenoid contents. In addition, TPT-induced total antioxidant capacities, the activities of superoxide dismutase and catalase, and the contents of malondialdehyde in liver and intestinal tissues indicated increases in oxidative stress. Both oxidative stress and coloration are linked to carotenoids. Thus, we speculated that the TPT-exposed males might use carotenoids to cope with increases in oxidative stress at the expense of carotenoid-based coloration. The disruption of mating behaviors and the decrease in attractiveness to females in male fish could result in reproductive failure. The present study underscores the importance of using behavioral tests as a sensitive tool in assessing the impact of pollutants present in aquatic environments.

由于三苯基锡（TPT）在水生生态系统中的广泛使用和普遍存在，因此对生态健康的影响备受关注。然而，关于TPT对鱼类繁殖行为的影响知之甚少。因此，本研究旨在研究环境相关浓度（0、1和10 ng Sn / L）下的TPT对雄性孔雀鱼（Poecilia reticulata）的交配行为和对雌性的吸引力的影响。）。结果表明，TPT暴露会干扰交配行为。暴露于TPT的雄鱼进行了更多的潜行尝试，但乙状结肠求爱没有变化。偷偷尝试的增加可能与TPT暴露引起的睾丸激素水平增加有关。在雄性竞争的情况下，暴露于TPT的雄性在交配过程中对雌性的吸引力较小。吸引力的降低可能与基于类胡萝卜素的色素减少有关，表现为尾鳍发红值和皮肤类胡萝卜素含量的降低。此外，TPT诱导的总抗氧化能力，超氧化物歧化酶和过氧化氢酶的活性以及肝和肠组织中丙二醛的含量表明氧化应激增加。氧化应激和着色都与类胡萝卜素有关。因此，我们推测，暴露于TPT的雄性可能会使用类胡萝卜素来应对氧化应激的增加，而以基于类胡萝卜素的着色为代价。雄性鱼类的交配行为中断和对雌性的吸引力下降可能会导致生殖衰竭。本研究强调使用行为测试作为评估水生环境中污染物影响的敏感工具的重要性。