By using 500 kHz high frequency ultrasound (US), 3,3’-diaminobenzidine (DAB) was treated at pH 2 under air atmosphere. The US effect of DAB absorption at 280 nm was composed at 43 and 141 kHz for the detection of fluorescence. When 100 W of US power was used, the DAB absorption band decreased with exposure time. It was noted that, a new absorption broad band at 350 nm – 500 nm appeared only under 141 and 500 kHz. Also, fluorescence spectra of the treated DAB were measured when excited at 370 nm. Increasing US exposure time resulted to a broad emission band at 478 nm, indicating that 1H,1’H-5,5’-Bibenzotriazole (BBT) was produced by US exposure at 500 kHz. This product was confirmed by NMR spectroscopy to be a triazine ring formed from NH₂ proton in DAB, due to the formation of nitrite ions in the US aqueous medium at 500 kHz. Hence, DAB was degraded while BBT was formed.

通过使用500 kHz高频超声（US），在空气气氛下在pH 2下处理3,3'-二氨基联苯胺（DAB）。280 nm处DAB吸收的US效应是在43和141 kHz上构成的，用于检测荧光。当使用100 W的US功率时，DAB吸收带会随着曝光时间而降低。值得注意的是，仅在141和500 kHz下出现了在350 nm – 500 nm处的新吸收宽带。同样，当在370nm激发时，测量处理过的DAB的荧光光谱。美国暴露时间的增加导致在478 nm处出现较宽的发射带，表明在500 kHz的美国暴露下会产生1H，1'H-5,5'-联苯并三唑（BBT）。通过NMR光谱确认该产物为由NH ₂形成的三嗪环。DAB中的质子，是由于在美国水介质中以500 kHz的频率形成了亚硝酸根离子。因此，DAB降解而形成BBT。