In this study, we report the simultaneous use of gold and silver nanoparticles to set a multicolor multiplex lateral flow immunoassay (xLFIA). Silver nanoparticles (AgNPs), spherical in shape and characterized by a brilliant yellow color, were obtained by a new viable one-step synthetic protocol. AgNPs were stable over time and acceptably robust to conditions used for fabricating LFIA devices. These AgNPs were employed as a colorimetric probe in combination with two different kinds of gold nanoparticles (AuNPs) to set a visual xLFIA for detecting allergens. Surface plasmon resonance peaks of probes (AgNPs, spherical and desert rose-like AuNPs) were centered at 420, 525, and 620 nm, respectively. Therefore, the xLFIA output was easily interpreted through a “yellow magenta cyan (YMC)” color code. The prospect of the YMC xLFIA was demonstrated by simultaneously detecting three major allergens in bakery products. Antibodies directed towards casein, ovalbumin, and hazelnut allergenic proteins were individually adsorbed onto metal nanoparticles to produce three differently colored specific probes. These were inserted in a LFIA comprising three lines, each responsive for one allergen. The trichromatic xLFIA was able to detect allergenic proteins at levels as low as 0.1 mg/l and enabled the easy identification of the allergens in commercial biscuits based on the color of the probes.

中文翻译：

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银和金纳米颗粒作为用于检测食物过敏原的多色侧向流动检测探针

在这项研究中，我们报告了同时使用金和银纳米颗粒来设置多色多重侧向免疫测定（xLFIA）。通过一种新的可行的一步合成方案，获得了球形，特征为亮黄色的银纳米颗粒（AgNP）。AgNP随时间稳定，并且对于制造LFIA器件所使用的条件具有可接受的鲁棒性。这些AgNPs与两种不同类型的金纳米颗粒（AuNPs）结合用作比色探针，以建立用于检测过敏原的可视化xLFIA。探针（AgNP，球形和沙漠玫瑰状AuNP）的表面等离子体共振峰分别位于420、525和620 nm处。因此，可以通过“黄色品红色青色（YMC）”颜色代码轻松地解释xLFIA输出。通过同时检测烘焙产品中的三种主要过敏原，证明了YMC xLFIA的前景。针对酪蛋白，卵清蛋白和榛子过敏原蛋白的抗体分别吸附到金属纳米颗粒上，以产生三种不同颜色的特异性探针。将它们插入包括三个系的LFIA中，每个系对一种变应原有反应。三色xLFIA能够以低至0.1 mg / l的水平检测过敏原蛋白，并能够根据探针的颜色轻松识别商用饼干中的过敏原。将它们插入包括三个系的LFIA中，每个系对一种变应原有反应。三色xLFIA能够以低至0.1 mg / l的水平检测过敏原蛋白，并能够根据探针的颜色轻松识别商用饼干中的过敏原。将它们插入包括三个系的LFIA中，每个系对一种变应原有反应。三色xLFIA能够以低至0.1 mg / l的水平检测过敏原蛋白，并能够根据探针的颜色轻松识别商用饼干中的过敏原。