当前位置: X-MOL 学术Phytochemistry › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Inoculation of Rhizoglomus irregulare or Trichoderma atroviride differentially modulates metabolite profiling of wheat root exudates
Phytochemistry ( IF 3.8 ) Pub Date : 2019-01-01 , DOI: 10.1016/j.phytochem.2018.10.033
Luigi Lucini , Giuseppe Colla , Maria Begoña Miras Moreno , Letizia Bernardo , Mariateresa Cardarelli , Valeria Terzi , Paolo Bonini , Youssef Rouphael

Root exudation patterns are linked to, among other things, plant growth, plant-microbe interaction and the priming effect. In this work, two complementary metabolomic approaches (both liquid and gas chromatography coupled to mass spectrometry) were applied to investigate the modulation of root exudation imposed by two beneficial fungi (substrate treatment of Trichoderma atroviride AT10, substrate application of Rhizoglomus irregulare BEG72 and seed treatment with T. atroviride AT10) on wheat (Triticum aestivum L.). The inoculation with R. irregulare elicited significant increases (by 18%, 39% and 20%) in the shoot, root dry biomass and root-to-shoot ratio compared to untreated plants, whereas inoculation with T. atroviride, as a substrate drench or as a seed coating, exhibited intermediate values for these parameters. The metabolomic approach demonstrated a broad chemical diversity, with more than 2900 compounds annotated in the root exudates. Overall, the Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) supervised modelling highlighted a distinctive modulation of the metabolic profile in the root exudates as a function of both fungal inoculation and means of application. Most of the differences could be ascribed to lipids (sterols and membrane lipids), phenolic compounds and terpenoids, siderophores and chelating acids, derivatives of amino acids and phytohormones, and as such, the interaction between the wheat roots and beneficial fungi resulted in a complex response in terms of root exudates, likely involving a cascade of processes. Nonetheless, the changes imposed by plant-microbe interactions can contribute to the support of the biostimulant effects of both T. atroviride and R. irregulare.

中文翻译:

接种不规则根霉或木霉属 atroviride 差异调节小麦根系分泌物的代谢物分析

根系分泌模式与植物生长、植物-微生物相互作用和启动效应等有关。在这项工作中,应用两种互补的代谢组学方法(液相和气相色谱与质谱联用)来研究两种有益真菌对根系分泌的调节(木霉属 atroviride AT10 的底物处理、不规则根霉 BEG72 的底物应用和种子处理)与小麦 (Triticum aestivum L.) 上的 T. atroviride AT10)。与未经处理的植物相比,接种不规则罗非鱼引起地上部、根干生物量和根对地比的显着增加(18%、39% 和 20%),而接种阿曲病毒作为基质浸液或作为种子包衣,显示这些参数的中间值。代谢组学方法展示了广泛的化学多样性,在根系分泌物中标注了 2900 多种化合物。总体而言,潜在结构判别分析的正交投影 (OPLS-DA) 监督建模突出了根系分泌物中代谢特征的独特调节,作为真菌接种和应用方式的函数。大多数差异可归因于脂质(甾醇和膜脂)、酚类化合物和萜类化合物、铁载体和螯合酸、氨基酸衍生物和植物激素,因此,小麦根和有益真菌之间的相互作用导致了复杂的根系分泌物的反应,可能涉及一系列过程。尽管如此,
更新日期:2019-01-01
down
wechat
bug