While triphenyl phosphate (TPhP) has been frequently detected in surface water and wildlife, its adverse effects on the gonadal development and reproductive behaviors of fish remain unclear. In this study, Japanese medaka (Oryzias latipes) were exposed to TPhP at concentrations of 134.1, 299.1, and 1429.5 ng/L from hatching [0 days posthatching (dph)] to sexual maturity (100 dph). TPhP induced gonadal intersex in male medaka in all exposure groups, and a significant increase was observed in the 1429.5 ng/L exposure group, with an incidence of 26.2% (11 of 42; p < 0.01). TPhP exposure also caused abnormal chasing behavior, with a lowest observable effective concentration (LOEC) of 299.1 ng/L, and reduced the desire of males for females in the 1429.5 ng/L group, demonstrating toxicity for fish reproductive behaviors. The anti-androgenic activity of TPhP via both androgen suppression and AR blocking was proposed to be the major mechanism of the observed effects. On the basis of dose-dependent inhibition of successful mating, fertilization, and hatching of eggs, environmentally relevant concentrations of TPhP pose a risk to fish reproduction.

中文翻译：

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与环境相关的有机磷阻燃剂磷酸三苯酯损害了日本Medaka（Oryzias latipes）的睾丸发育和生殖行为。

尽管经常在地表水和野生动植物中检测到磷酸三苯酯（TPhP），但尚不清楚其对鱼的性腺发育和繁殖行为的不利影响。在这项研究中，从孵化[孵化后0天（dph）]到性成熟（100 dph），日本（Oryzias latipes）暴露于TPhP的浓度为134.1、299.1和1429.5 ng / L。TPhP在所有暴露组的雄性med中诱导性腺性交，在1429.5 ng / L暴露组中观察到显着增加，发生率为26.2％（42中的11；p<0.01）。TPhP暴露也引起异常的追捕行为，最低可观察到有效浓度（LOEC）为299.1 ng / L，并降低了1429.5 ng / L组中男性对女性的渴望，证明了对鱼类生殖行为的毒性。TPhP通过抑制雄激素和阻断AR的抗雄激素活性被认为是观察到的作用的主要机理。根据剂量对成功交配，受精和孵化卵的抑制作用，与​​环境相关的TPhP浓度对鱼类繁殖构成了风险。