当前位置: X-MOL 学术Biochimie › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Silencing of LncRNA steroid receptor RNA activator attenuates polycystic ovary syndrome in mice
Biochimie ( IF 3.9 ) Pub Date : 2018-11-01 , DOI: 10.1016/j.biochi.2018.10.021
Yan Li , Wanqiu Zhao , Haixu Wang , Chen Chen , Dongmei Zhou , Shengnan Li , Xiaohong Zhang , Haibo Zhao , Dangxia Zhou , Biliang Chen

Background

Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women of reproductive age and has a prevalence of 1 in 15 women worldwide. This study aims to investigate the role of lncRNA SRA in the pathological processes of polycystic ovary syndrome (PCOS).

Methods

Twenty five-day old female C57BL/6 mice received subcutaneous injection of 60 mg/kg dehydroepiandrosterone for 20 days to induce PCOS. Lentivirus containing lncRNA SRA-specific shRNA was subcapsularly injected into the ovaries of PCOS mice. Granulosa cell was primary cultured to explore the mechanism of DHEA-induced inflammatory responses. H&E staining was used to examine the histological changes of ovaries. ELISA was used to assess serum insulin level and proinflammatory cytokines and angiogenetic factors contents in ovary tissue. The expression levels of LncRNA SRA and proteins involved in the NF-κB signaling pathway were detected through Quantitative real-time PCR and Western blot. The nuclear translocation of NF-κB was observed by immunofluorescence and the activity of NF-κB-DNA binding was detected using EMSA.

Results

Silencing of lncRNA SRA changed insulin release, attenuated ovary injury and reduced the production of angiogenetic factors in the PCOS mice. In addition, shRNA targeting lncRNA SRA inhibited DHEA-induced pro-inflammatory cytokines production and NF-κB nuclear translocation in the ovary of PCOS mice and primary granulosa cells.

Conclusion

Silencing of lncRNA Steroid Receptor RNA Activator (SRA) attenuates polycystic ovary syndrome (PCOS) in mice. LncRNA SRA plays important roles in the development of PCOS.



中文翻译:

沉默LncRNA类固醇受体RNA激活剂可减轻小鼠多囊卵巢综合征

背景

多囊卵巢综合征(PCOS)是育龄妇女中最常见的内分泌失调,全世界15个妇女中有1个患病率。这项研究旨在调查lncRNA SRA在多囊卵巢综合征(PCOS)病理过程中的作用。

方法

二十五天大的雌性C57BL / 6小鼠接受皮下注射60 mg / kg脱氢表雄酮20天,以诱导PCOS。将含有lncRNA SRA特异性shRNA的慢病毒皮下注射到PCOS小鼠的卵巢中。原代培养颗粒细胞以探索DHEA诱导的炎症反应的机制。H&E染色用于检查卵巢的组织学变化。ELISA用于评估卵巢组织中的血清胰岛素水平和促炎细胞因子以及血管生成因子的含量。通过实时荧光定量PCR和Western blot检测LncRNA SRA和NF-κB信号通路蛋白的表达水平。通过免疫荧光观察NF-κB的核易位,并使用EMSA检测NF-κB-DNA结合的活性。

结果

沉默lncRNA SRA可以改变PCOS小鼠的胰岛素释放,减轻卵巢损伤并减少血管生成因子的产生。此外,靶向lncRNA SRA的shRNA抑制了PCOS小鼠和原代颗粒细胞卵巢中DHEA诱导的促炎性细胞因子的产生和NF-κB核易位。

结论

lncRNA类固醇受体RNA激活剂(SRA)的沉默可减轻小鼠多囊卵巢综合征(PCOS)。LncRNA SRA在PCOS的发展中起着重要作用。

更新日期:2018-11-01
down
wechat
bug