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Cutinases catalyze polyacrylate hydrolysis and prevent their aggregation
Polymer Degradation and Stability ( IF 5.9 ) Pub Date : 2018-10-31 , DOI: 10.1016/j.polymdegradstab.2018.10.028
Ruoyu Hong , Lingqia Su , Jing Wu

During the recycling of waste paper, the accumulation of polyacrylates present in the waste paper causes the formation of tacky substances known as stickies. Deposition of these stickies on the machinery decreases the quality of the recycled paper and increases the usage of circulating water. Enzymes that hydrolyze these polyacrylates can minimize or eliminate the deposition of stickies. In initial experiments, the abilities of the cutinases from Humicola insolens, Fusarium solani and Thermobifida fusca to hydrolyze poly (methyl acrylate) (PMA) and poly (ethyl acrylate) (PEA) within a macroporous resin were compared. Then, to simulate the environment encountered during paper recycling, PMA and PEA dispersions were used as substrates. The decrease in turbidity was measured at a concentration of 0.5 mg mL−1. When used at pH 8.0 and 30–50 °C, T. fusca cutinase limited the turbidity decrease to about 1.0% and favored the hydrolysis of PEA over PMA. F. solani and H. insolens cutinases performed best at pH 8.5 and temperatures of 35 and 50 °C, respectively. At a polyacrylate concentration of 0.1 mg mL−1, the optimal temperatures of these cutinases decreased. The optimal T. fusca cutinase dosage was lower than those of F. solani and H. insolens cutinases.



中文翻译:

角质酶催化聚丙烯酸酯水解并防止其聚集

在废纸回收期间,废纸中存在的聚丙烯酸酯堆积会导致形成发粘物质,称为胶粘物。这些胶粘物在机器上的沉积会降低再生纸的质量,并增加循环水的使用量。水解这些聚丙烯酸酯的酶可以减少或消除胶粘物的沉积。在最初的实验中,来自腐植质腐质霉茄枯萎病菌深热的角质酶的能力比较了在大孔树脂中水解聚丙烯酸酯(PMA)和聚丙烯酸酯(PEA)的方法。然后,为了模拟纸张回收期间遇到的环境,将PMA和PEA分散体用作基材。在0.5 mg mL -1的浓度下测得浊度降低。当在pH 8.0和30–50°C下使用时,T。fusca角质酶将浊度限制在约1.0%,并且比PEA更有利于PEA的水解。F. solaniH. insolens角质酶分别在pH 8.5和35和50°C的温度下表现最佳。在聚丙烯酸酯浓度为0.1 mg mL -1时,这些角质酶的最佳温度降低。最佳T. fusca角质酶的剂量低于F. solaniH. insolens角质酶的剂量。

更新日期:2018-10-31
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