当前位置: X-MOL 学术Food Hydrocoll. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Biocompatible polymeric nanoparticles with exceptional gastrointestinal stability as oral delivery vehicles for lipophilic bioactives
Food Hydrocolloids ( IF 10.7 ) Pub Date : 2019-04-01 , DOI: 10.1016/j.foodhyd.2018.10.057
Qiaobin Hu , Minkyung Bae , Erika Fleming , Ji-Young Lee , Yangchao Luo

Abstract Poor stability in gastrointestinal (GI) tract is a major challenge for applications of natural biopolymer-based nanoparticles as oral delivery vehicles. In this study, chitosan was modified by grafting stearic acid on its backbone to improve its amphiphilicity. The polymeric nanoparticles were prepared using stearic acid-conjugated chitosan and sodium caseinate via ionic gelation and chemical crosslinking by glutaraldehyde and periodate oxidized dextran. The physicochemical properties and stability in simulated GI fluids were systematically studied to obtain the optimal nanoparticles with a diameter of 150 nm and homogeneous size distribution. The electron microscope images suggested the well-maintained nanoparticulate structure in simulated GI fluids. The non-cytotoxicity and internalization of the nanoparticles by Caco-2 cells were confirmed using fluorescence microscope. Successful encapsulation and sustained release of curcumin were demonstrated. The as-prepared polymeric nanoparticles hold promising features to be oral delivery vehicles to carry lipophilic bioactives for future applications in food and pharmaceutical industries.

中文翻译:

具有卓越胃肠道稳定性的生物相容性聚合物纳米粒子作为亲脂性生物活性物质的口服递送载体

摘要 胃肠 (GI) 道稳定性差是基于天然生物聚合物的纳米粒子作为口服递送载体的应用的主要挑战。在这项研究中,壳聚糖通过在其骨架上接枝硬脂酸来改进其两亲性。通过离子凝胶化和戊二醛和高碘酸氧化葡聚糖的化学交联,使用硬脂酸偶联壳聚糖和酪蛋白酸钠制备聚合物纳米颗粒。系统地研究了模拟胃肠液中的理化性质和稳定性,以获得直径为 150 nm 且尺寸分布均匀的最佳纳米粒子。电子显微镜图像表明模拟 GI 流体中维护良好的纳米颗粒结构。使用荧光显微镜证实了 Caco-2 细胞对纳米颗粒的非细胞毒性和内化。证明了姜黄素的成功包封和持续释放。所制备的聚合物纳米粒子具有作为口服递送载体的有希望的特征,可携带亲脂性生物活性物质,用于食品和制药行业的未来应用。
更新日期:2019-04-01
down
wechat
bug