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Lipase synthesis of isoamyl acetate using different acyl donors: Comparison of novel esterification techniques
LWT - Food Science and Technology ( IF 6 ) Pub Date : 2018-11-01 , DOI: 10.1016/j.lwt.2018.10.098
Mahdieh Zare , Mohammad-Taghi Golmakani , Mehrdad Niakousari

The present work focused on solvent-free microwave-assisted esterification (MAE) of isoamyl acetate. It is based on the hypothesis that isoamyl acetate yield can be affected by variables such as different acyl donors (acetic acid, acetic anhydride, and ethyl acetate), lipases (Novozyme 435 and Lipozyme RMIM) and microwave power levels (100 and 200 W). Furthermore, this study aimed to make comparisons between MAE and other esterification techniques, namely the ohmic, ultrasound, and incubator. Maximum isoamyl acetate yield (100%) was obtained at a power level of 100 W in MAE after 1 h when acetic anhydride and Novozyme 435 were used in the process. MAE had the lowest specific CO2 emission (16 g/g isoamyl acetate) and specific electric energy consumption (0.02 kWh/g isoamyl acetate) in comparison with other esterification techniques. The MAE had no adverse effects on the structure of Novozyme 435 beads according to scanning electron microscopy images. After using the same Novozyme 435 repeatedly for 7 times, the yield of isoamyl acetate became lower than 19%. In conclusion, MAE can be proposed as a green technique for the lipase esterification of different flavoring esters.



中文翻译:

使用不同的酰基供体合成乙酸异戊酯的脂肪酶:新型酯化技术的比较

目前的工作集中在乙酸异戊酯的无溶剂微波辅助酯化(MAE)上。基于这样的假设,乙酸异戊酯的收率可能受到变量的影响,例如不同的酰基供体(乙酸,乙酸酐和乙酸乙酯),脂肪酶(Novozyme 435和Lipozyme RMIM)和微波功率水平(100和200 W) 。此外,本研究旨在对MAE和其他酯化技术(即欧姆,超声和培养箱)进行比较。当在该过程中使用乙酸酐和Novozyme 435时,在1 h后在MAE中以100 W的功率水平获得最大乙酸异戊酯产率(100%)。MAE的最低CO 2最低与其他酯化技术相比,其排放量(16 g / g乙酸异戊酯)和特定的电能消耗(0.02 kWh / g乙酸异戊酯)。根据扫描电子显微镜图像,MAE对Novozyme 435磁珠的结构没有不利影响。重复使用相同的Novozyme 435 7次后,乙酸异戊酯的收率低于19%。总之,MAE可以作为一种绿色技术用于不同风味酯的脂肪酶酯化。

更新日期:2018-11-01
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