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Radiolabeled Molecular Imaging Probes for the In Vivo Evaluation of Cellulose Nanocrystals for Biomedical Applications.
Biomacromolecules ( IF 6.2 ) Pub Date : 2018-11-09 , DOI: 10.1021/acs.biomac.8b01313
Surachet Imlimthan 1 , Sofia Otaru 1 , Outi Keinänen 1 , Alexandra Correia 2 , Kalle Lintinen 3 , Hélder A Santos 2, 4 , Anu J Airaksinen 1 , Mauri A Kostiainen 3 , Mirkka Sarparanta 1
Affiliation  

Cellulose nanocrystals (CNCs) have remarkable potential to improve the delivery of diagnostic and therapeutic agents to tumors; however, the in vivo studies on CNC biodistribution are still limited. We developed CNC-based imaging probes for the in vitro and in vivo evaluation using two labeling strategies: site-specific hydrazone linkage to the terminal aldehyde of the CNC and nonsite-specific activation using 1,1'-carbonyldiimidazole (CDI). The in vivo behavior of unmodified CNC, DOTA-CNC (ald.), and DOTA-CNC (OH) was investigated in healthy and 4T1 breast cancer mouse models. They displayed good biocompatibility in cell models. Moreover, the biodistribution profile and SPECT/CT imaging confirmed that the accumulation of 111In-labeled DOTA-CNC (ald.) and 111In-DOTA-CNC (OH) was primarily in hepatic, splenic, and pulmonary ducts in accordance with the clearance of nontargeted nanoparticles. The developed CNC imaging probes can be used to obtain information with noninvasive imaging on the behavior in vivo to guide structural optimization for targeted delivery.

中文翻译:

用于生物医学应用的纤维素纳米晶体体内评估的放射性标记分子成像探针。

纤维素纳米晶体(CNCs)具有显着的潜力,可以改善诊断和治疗药物对肿瘤的输送。然而,体内对CNC生物分布的研究仍然有限。我们开发了基于CNC的成像探针,用于通过两种标记策略进行体外和体内评估:位点特异性与CNC末端醛的键合以及非位点特异性的使用1,1'-羰基二咪唑(CDI)的激活。在健康和4T1乳腺癌小鼠模型中研究了未修饰的CNC,DOTA-CNC(ald。)和DOTA-CNC(OH)的体内行为。他们在细胞模型中显示出良好的生物相容性。此外,生物分布图和SPECT / CT成像证实111In标记的DOTA-CNC(ald。)和111In-DOTA-CNC(OH)的积累主要在肝,脾,并根据非靶向纳米粒子的清除率确定肺管。所开发的CNC成像探头可用于通过无创成像获得有关体内行为的信息,以指导针对靶向递送的结构优化。
更新日期:2018-11-01
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