The modification of rhizosphere microbial diversity and ecological processes are of rising interest as shifting in microbial community structure impacts the mutual role of host–microbe interactions. Nevertheless, the connection between host-microbial community diversity, their function under biotic stress in addition to their impact on plant performances is poorly understood. The study was designed with the aim to analyze the tripartite interactions among Chitiniphilus sp., Streptomyces sp. and their combination with indigenous rhizospheric microbial population of Bacopa monnieri for enhancing the plant growth and bacoside A content under Meloidogyne incognita stress. Overall, plants treated with the microbial combination recorded enhanced growth as illustrated by significantly higher biomass (2.0 fold), nitrogen uptake (1.8 fold) and bacoside A content (1.3 fold) along with biocontrol efficacy (58.5%) under nematode infected field. The denaturing gradient gel electrophoresis (DGGE) fingerprints of 16S-rDNA revealed that microbial inoculations are major initiators of bacterial community structure in the plant rhizosphere. Additionally, the plants treated with microbial combination showed maximum diversity viz., Shannon’s (3.29), Margalef’s (4.21), and Simpson’s (0.96) indices. Likewise the metabolic profiling data also showed a significant variation among the diversity and evenness indices upon microbial application on the native microflora. We surmise that the application of beneficial microbes in combinational mode not only helped in improving the microbial community structure but also successfully enhanced plant and soil health under biotic stress.

随着微生物群落结构的变化影响宿主-微生物相互作用的相互作用，根际微生物多样性和生态过程的改变引起了人们的浓厚兴趣。然而，人们对宿主-微生物群落多样性，它们在生物胁迫下的功能以及对植物性能的影响之间的联系知之甚少。设计该研究的目的是分析Chitiniphilus sp。，Streptomyces sp。之间的三方相互作用。及其与本地根际微生物Bacopa monnieri的组合，以提高根结线虫下植物的生长和Bacoside A的含量压力。总体而言，用微生物组合物处理的植物记录到生长增强，这表明在线虫感染的田间，生物量（2.0倍），氮素吸收（1.8倍）和ba子苷A含量（1.3倍）显着提高，以及生物防治功效（58.5％）。16S-rDNA的变性梯度凝胶电泳（DGGE）指纹图谱表明，微生物接种是植物根际细菌群落结构的主要引发剂。另外，用微生物组合处理的植物表现出最大的多样性，即Shannon（3.29），Margalef（4.21）和Simpson（0.96）指数。同样，当对天然菌群进行微生物施用时，代谢谱数据也显示出多样性和均匀度指数之间的显着差异。