Research into post-transcriptional processing and modification of RNA continues to speed forward, as their ever-emerging role in the regulation of gene expression in biological systems continues to unravel. Liquid chromatography tandem mass spectrometry (LC-MS/MS) has proven for over two decades to be a powerful ally in the elucidation of RNA modification identity and location, but the technique has not proceeded without its own unique technical challenges. The throughput of LC-MS/MS modification mapping experiments continues to be impeded by tedious and time-consuming spectral interpretation, particularly during for the analysis of complex RNA samples. RNAModMapper was recently developed as a tool to improve the interpretation and annotation of LC-MS/MS data sets from samples containing post-transcriptionally modified RNAs. Here, we delve deeper into the methodology and practice of RNAModMapper to provide greater insight into its utility, and remaining hurdles, in current RNA modification mapping experiments.

中文翻译：

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改进了 RNAModMapper（一种 RNA 修饰映射软件工具）的应用，用于分析液相色谱串联质谱 (LC-MS/MS) 数据

对 RNA 的转录后加工和修饰的研究继续加速推进，因为它们在生物系统中基因表达的调节中不断出现的作用不断被揭示。二十多年来，液相色谱串联质谱 (LC-MS/MS) 已被证明是阐明 RNA 修饰身份和位置的强大盟友，但该技术的发展也面临着独特的技术挑战。LC-MS/MS 修饰映射实验的吞吐量继续受到繁琐和耗时的光谱解释的阻碍，尤其是在分析复杂 RNA 样品期间。RNAModMapper 最近被开发为一种工具，用于改进对含有转录后修饰 RNA 的样品的 LC-MS/MS 数据集的解释和注释。这里，