Abstract Debranched starches (DBSs) are widely used in food, pharmaceuticals, and health products. The purpose of this study is to separate fractions of debranched normal starch with different degrees of polymerization (DP). Five fractions were obtained by the ethanol precipitation fractionation method with different water-to-ethanol ratios, including a very long DBSp (p: precipitate), long DBSp1.5 (p1.5: water/ethanol = 1:1.5 precipitate), moderately long DBSp4 (p4: water/ethanol = 1:4 precipitate), short DBSs1.5 (s1.5: water/ethanol = 1:1.5 supernatant), and very short DBSs4 (s4: water/ethanol = 1:4 supernatant). The main peak DP values of different fractions of debranched normal corn starch were 24.41, 16.74, 13.70, 7.77, and 6.09, respectively. The fractions of DBSs from potato and sweet potato starches had larger molecular weights. The crystalline structure of DBSp and DPSp4 was B-type while that of DBSp1.5 was A-type. DBSs with different properties could be obtained from normal starch by the green and simple aqueous ethanol precipitation method.

中文翻译：

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从普通玉米、马铃薯和甘薯淀粉中脱支的线性葡聚糖的分离和表征

摘要 脱支淀粉（DBSs）广泛应用于食品、药品和保健品中。本研究的目的是分离具有不同聚合度 (DP) 的脱支正常淀粉部分。通过乙醇沉淀分馏法获得了五个不同水乙醇比的馏分，包括很长的 DBSp（p：沉淀）、长的 DBSp1.5（p1.5：水/乙醇 = 1:1.5 沉淀）、中等长 DBSp4（p4：水/乙醇 = 1:4 沉淀物）、短 DBSs1.5（s1.5：水/乙醇 = 1:1.5 上清液）和非常短的 DBSs4（s4：水/乙醇 = 1:4 上清液） . 脱支正常玉米淀粉不同级分的主峰DP值分别为24.41、16.74、13.70、7.77和6.09。来自马铃薯和甘薯淀粉的 DBS 级分具有较大的分子量。DBSp和DPSp4的晶体结构为B型，而DBSp1.5的晶体结构为A型。通过绿色简单的乙醇水溶液沉淀法，可以从普通淀粉中获得不同性质的DBSs。