Nuclear morphology is indicative of cellular health in many contexts. In order to robustly and quantitatively measure nuclear size and shape, numerous experimental methods leveraging fluorescence microscopy have been developed. While these methods are useful for quantifying two-dimensional morphology, they often fail to accurately represent the three-dimensional structure of the nucleus, thus omitting important spatial and volumetric information. To address the need for a more accurate image analysis modality, we have developed a software platform that faithfully reconstructs membrane surfaces in three dimensions with sub-pixel resolution. Here, we showcase its broad applicability across species and nuclear scale, as well as provide information on how to employ this platform for diverse experimental systems.

中文翻译：

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用于三维核重建的多功能图像分析平台

在许多情况下，核形态是细胞健康的指标。为了稳健和定量地测量核的大小和形状，已经开发了许多利用荧光显微镜的实验方法。虽然这些方法可用于量化二维形态，但它们往往无法准确表示细胞核的三维结构，从而遗漏了重要的空间和体积信息。为了满足对更准确的图像分析模式的需求，我们开发了一个软件平台，可以在三个维度上以亚像素分辨率忠实地重建膜表面。在这里，我们展示了它在物种和核尺度上的广泛适用性，并提供了有关如何将该平台用于各种实验系统的信息。