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Fruit Bagasse Phytochemicals from Malpighia Emarginata Rich in Enzymatic Inhibitor with Modulatory Action on Hemostatic Processes
Journal of Food Science ( IF 3.9 ) Pub Date : 2018-10-18 , DOI: 10.1111/1750-3841.14330
Tamara R. Marques 1 , Pedro Henrique S. Cesar 1 , Mariana A. Braga 1 , Silvana Marcussi 1 , Angelita D. Corrêa 1
Affiliation  

Agro-industrial wastes are promising sources of phytochemicals for the development of products to be used in health promotion and maintenance. In this study, extracts from acerola bagasse (AB) were characterized by HPLC, and evaluated according to its modulatory action on phospholipases A2 and proteases involved in processes such as inflammation and blood clotting. Snake venoms were used as biological tools once they have high functional and structural homology between their enzymes and human enzymes. Two types of extracts were prepared from AB: aqueous and methanolic. These extracts, evaluated at different proportions (venom:extract, w:w), significantly inhibited the phospholipase activity induced by the venoms of Bothrops moojeni, Bothrops atrox (11% to 31%), and Crotalus durissus terrificus (C. d. t.) (11% to 19%). The hemolytic activity induced by the venoms of B. moojeni and C. d. t. was better inhibited by the methanolic extract (inhibition between 23% and 48%). Thrombolysis induced by the venoms of B. moojeni and C. d. t. was inhibited by both extracts, with inhibition ranging from 13% to 63% for the aqueous extract, and from 12% to 92% for the methanolic one. Both extracts increased the time of coagulation induced by the venoms of B. moojeni and Lachesis muta muta in 26 and up to 68 s. These inhibitory actions were related to the following phenolic compounds present in the extract of AB: gallic acid, catechin, epigallocatechin gallate, epicatechin, syringic acid, p-coumaric acid, and quercetin. Additional studies are needed to confirm their potential use for nutraceutical purposes. PRACTICAL APPLICATION: Agro-industrial wastes are promising sources of phytochemicals for the development of products that can be used by pharmaceutical, cosmetics, and food industries. Studies report the use of the acerola bagasse extract in health improvement. However, its toxic-pharmacological characterization is still scarce. In this study, the extracts of acerola bagasse presented phenolic compounds that can modulate the activity of enzymes such as phospholipases A2 and proteases that act on the coagulant/anticoagulant and thrombotic/thrombolytic activities and the break of phospholipids, decreasing the inflammation and platelet aggregation. Although the in vivo effects of the extracts are not fully understood, this study shed light upon the possibilities of their usage.

中文翻译:

来自 Malpighia Emarginata 的水果甘蔗渣植物化学物质富含酶抑制剂,对止血过程具有调节作用

农业工业废物是用于开发用于健康促进和维护的产品的植物化学物质的有希望的来源。在这项研究中,针叶樱桃渣 (AB) 的提取物通过 HPLC 进行表征,并根据其对磷脂酶 A2 和蛋白酶的调节作用进行评估,这些蛋白酶参与炎症和凝血等过程。一旦蛇毒的酶和人类酶之间具有高度的功能和结构同源性,蛇毒就被用作生物工具。从 AB 制备两种类型的提取物:水相和甲醇相。这些提取物以不同的比例(毒液:提取物,w:w)进行评估,显着抑制了由 Bothrops moojeni、Bothrops atrox(11% 至 31%)和 Crotalus durissus terrificus (C. dt) 毒液诱导的磷脂酶活性( 11% 到 19%)。B. moojeni 和 C. dt 毒液诱导的溶血活性被甲醇提取物更好地抑制(抑制在 23% 和 48% 之间)。B. moojeni 和 C. dt 毒液诱导的血栓溶解被两种提取物抑制,水提取物的抑制范围为 13% 至 63%,甲醇提取物的抑制范围为 12% 至 92%。两种提取物都将 B. moojeni 和 Lachesis muta muta 毒液诱导的凝固时间增加了 26 秒,最长可达 68 秒。这些抑制作用与 AB 提取物中存在的以下酚类化合物有关:没食子酸、儿茶素、表没食子儿茶素没食子酸酯、表儿茶素、丁香酸、对香豆酸和槲皮素。需要额外的研究来确认它们在营养保健品方面的潜在用途。实际应用:农业工业废物是用于开发可用于制药、化妆品和食品工业的产品的植物化学物质的有前途的来源。研究报告了针叶樱桃甘蔗渣提取物在改善健康方面的用途。然而,其毒性药理学表征仍然很少。在这项研究中,针叶樱桃渣的提取物含有酚类化合物,可以调节酶的活性,如磷脂酶 A2 和蛋白酶,这些酶作用于凝血/抗凝和血栓/血栓溶解活性以及磷脂的分解,减少炎症和血小板聚集。虽然提取物的体内作用尚未完全了解,但这项研究揭示了它们使用的可能性。和食品工业。研究报告了针叶樱桃甘蔗渣提取物在改善健康方面的用途。然而,其毒性药理学表征仍然很少。在这项研究中,针叶樱桃渣的提取物含有酚类化合物,可以调节酶的活性,如磷脂酶 A2 和蛋白酶,这些酶作用于凝血/抗凝和血栓/血栓溶解活性以及磷脂的分解,减少炎症和血小板聚集。虽然提取物的体内作用尚未完全了解,但这项研究揭示了它们使用的可能性。和食品工业。研究报告了针叶樱桃甘蔗渣提取物在改善健康方面的用途。然而,其毒性药理学表征仍然很少。在这项研究中,针叶樱桃渣的提取物含有酚类化合物,可以调节酶的活性,如磷脂酶 A2 和蛋白酶,这些酶作用于凝血/抗凝和血栓/血栓溶解活性以及磷脂的分解,减少炎症和血小板聚集。虽然提取物的体内作用尚未完全了解,但这项研究揭示了它们使用的可能性。针叶樱桃甘蔗渣的提取物含有酚类化合物,可以调节酶的活性,例如磷脂酶 A2 和蛋白酶,这些酶作用于凝血/抗凝和血栓/血栓溶解活性以及磷脂的分解,从而减少炎症和血小板聚集。虽然提取物的体内作用尚未完全了解,但这项研究揭示了它们使用的可能性。针叶樱桃甘蔗渣的提取物含有酚类化合物,可以调节酶的活性,例如磷脂酶 A2 和蛋白酶,这些酶作用于凝血/抗凝和血栓/血栓溶解活性以及磷脂的分解,从而减少炎症和血小板聚集。虽然提取物的体内作用尚未完全了解,但这项研究揭示了它们使用的可能性。
更新日期:2018-10-18
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