Oocyte and embryo developmental competence are shaped by multiple extrinsic and intrinsic factors. One of the most extensive research areas in the last decade is the regulation of lipid metabolism in oocytes and embryos of different species. We hypothesized that differences in developmental competence of oocytes and embryos between prepubertal and cyclic gilts may arise due to distinct fatty acid profiles in follicular fluid. We found that supplementation of oocyte maturation media with follicular fluid from prepubertal pigs affected quality and development of embryos from prepubertal pigs while embryos of cyclic pigs were not affected. PLIN2, SCD and ACACA transcripts involved in lipid metabolism were upregulated in embryos originating from oocytes of prepubertal pigs matured with autologous follicular fluid. The surface occupied by lipid droplets tend to increase in oocytes matured with follicular fluid from prepubertal pigs regardless oocyte origin. The change into follicular fluid of cyclic pigs increased the efficiency of embryo culture and improved quality, while gene expression was similar to embryos obtained from cyclic gilts. We assume that the follicular fluids of prepubertal and cyclic pigs influenced the quality of oocytes and embryos obtained from prepubertal pigs which are more susceptible to suboptimal in vitro culture conditions.

中文翻译：

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补充卵泡液的猪IVM培养基的后果已反映在胚胎质量，产量和基因表达模式上。

卵母细胞和胚胎发育能力受多种外在和内在因素影响。过去十年中最广泛的研究领域之一是调节不同物种的卵母细胞和胚胎中的脂质代谢。我们假设青春期前和环状后备母猪在卵母细胞和胚胎发育能力上的差异可能是由于卵泡液中不同的脂肪酸谱引起的。我们发现，青春期前猪卵泡液补充卵母细胞成熟培养基会影响青春期前猪胚胎的质量和发育，而周期性猪的胚胎则不会受到影响。参与脂质代谢的PLIN2，SCD和ACACA转录本在自体卵泡液成熟的青春期前猪卵母细胞的胚胎中被上调。不论卵母细胞的来源如何，在来自青春期前猪卵泡液成熟的卵母细胞中，脂质滴所占据的表面往往会增加。周期性猪的卵泡液变化增加了胚胎培养的效率并提高了品质，而基因表达与从周期性后备母猪获得的胚胎相似。我们假设青春期前和周期猪的卵泡液会影响从青春期前猪获得的卵母细胞和胚胎的质量，这些卵母细胞和胚胎更容易受到次最佳体外培养条件的影响。