Quantification of superoxide anions (O₂⁻) is of great significance for the pathological research of many diseases. In this work, a non-enzymatic electrochemical sensor for superoxide sensing was fabricated on the basis of metal-organic frameworks (MOFs)-derived porous carbon matrix loaded with Pt and Co nanoparticles (denoted as Pt-Co-PC). Zeolitic imidazolate framework-67 (ZIF-67) was utilized as the precursor through a one-step calcination under N₂ atmosphere to prepare cobalt nanoparticles-porous carbon composites (denoted as Co-PC). Pt-Co-PC was then obtained by using a facile galvanic replacement procedure with Co-PC and chloroplatinic acid solution. Furthermore, the home-made screen printed gold electrodes (SPGEs) were used to immobilize the as-synthesized materials, constructing Pt-Co-PC modified electrochemical sensors. The recommended Pt-Co-PC/SPGE sensors exhibit excellent electrochemical performance for the detection of O₂⁻ with an extremely low detection limit of 0.118 μM (S/N = 3) and a high sensitivity of 6.218 × 10⁻⁴ mA μM⁻¹ cm⁻². Meanwhile, the proposed sensors possess superior reproducibility and stability. More importantly, our sensors have successfully captured the electrochemical signals of O₂⁻ released from Hela cells, which means the fabricated sensors have potential application of monitoring O₂⁻ in pathological and biological fields.

超氧阴离子的定量（O ₂ ^- ）是许多疾病的病理研究具有十分重要的意义。在这项工作中，基于负载有Pt和Co纳米颗粒（表示为Pt-Co-PC）的金属有机框架（MOFs）衍生的多孔碳基质，制造了用于超氧化物传感的非酶电化学传感器。通过在N ₂下一步煅烧将沸石咪唑盐骨架67（ZIF-67）用作前体在大气中制备钴纳米颗粒-多孔碳复合材料（称为Co-PC）。然后，通过使用Co-PC和氯铂酸溶液的简便电置换程序获得Pt-Co-PC。此外，使用自制的丝网印刷金电极（SPGE）固定合成后的材料，构建了Pt-Co-PC修饰的电化学传感器。推荐的铂-钴- PC / SPGE传感器表现出优良的电化学性能，用于检测的O- ₂ ^-与0.118μM（S / N = 3）和6.218×10高灵敏度极低的检测极限^-4 毫安μM ^{- 1} 厘米^-2。同时，提出的传感器具有优异的再现性和稳定性。更重要的是，我们的传感器已经成功捕获的O-电化学信号₂ ^-从Hela细胞，这意味着所制造的传感器具有监测的O潜在的应用释放₂ ^-在病理和生物学领域。