Electrospun natural polymeric bandages are highly desirable due to their low-cost, biodegradability, non-toxicity and antimicrobial properties. Functionalization of these nanofibrous mats with two-dimensional nanomaterials is an attractive strategy to enhance the antibacterial effects. Herein, we demonstrate an electrospinning process to produce encapsulated delaminated Ti₃C₂T_z (MXene) flakes within chitosan nanofibers for passive antibacterial wound dressing applications. In vitro antibacterial studies were performed on crosslinked Ti₃C₂T_z/chitosan composite fibers against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) – demonstrating a 95% and 62% reduction in colony forming units, respectively, following 4 h of treatment with the 0.75 wt% Ti₃C₂T_z – loaded nanofibers. Cytotoxicity studies to determine biocompatibility of the nanofibers indicated the antibacterial MXene/chitosan nanofibers are non-toxic. The incorporation of Ti₃C₂T_z single flakes on fiber morphology was analyzed by scanning electron microscopy (SEM) and transmission electron microscopy equipped with an energy-dispersive detector (TEM-EDS). Our results suggest that the electrospun Ti₃C₂T_z/chitosan nanofibers are a promising candidate material in wound healing applications.

中文翻译：

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静电纺丝 Ti3C2Tz (MXene)/壳聚糖纳米纤维的抗菌性能†

电纺天然聚合物绷带由于其低成本、可生物降解、无毒和抗菌特性而非常受欢迎。用二维纳米材料对这些纳米纤维垫进行功能化是增强抗菌效果的一种有吸引力的策略。在此，我们展示了一种静电纺丝工艺，用于在壳聚糖纳米纤维中生产封装的分层 Ti ₃ C ₂ T _z (MXene) 薄片，用于被动抗菌伤口敷料应用。_{交联Ti 3} C ₂ T _z /壳聚糖复合纤维对革兰氏阴性大肠杆菌（E.coli ）的体外抗菌研究) 和革兰氏阳性金黄色葡萄球菌( S. aureus ) – 在用 0.75 wt% Ti ₃ C ₂ T _z负载的纳米纤维处理 4 小时后，菌落形成单位分别减少了 95% 和 62% 。确定纳米纤维生物相容性的细胞毒性研究表明抗菌 MXene/壳聚糖纳米纤维是无毒的。通过扫描电子显微镜 (SEM) 和配备能量色散检测器 (TEM-EDS) 的透射电子显微镜分析Ti ₃ C ₂ T _{z单片在纤维形态上的掺入。}我们的结果表明，静电纺丝 Ti ₃ C ₂T _z /壳聚糖纳米纤维是伤口愈合应用中很有前途的候选材料。