A solid-state electrochemiluminescence (ECL) biosensor for the detection of concanavalin A (Con A) was constructed using graphene quantum dots (GQDs) as luminophore, which were loaded on polyamidoamine (PAMAM) functionalized CeO₂@Ag NPs by the interaction between the NH₂ of PAMAM and the COOH of GQDs. The electrodepositing gold layers were used as matrix to immobilize glucose oxidase (GOx). GOx served as recognition elements to further capture Con A by the specific interaction between carbohydrate and Con A. With the increase of the concentration of Con A, the amount of GOx-CeO₂@Ag-GQDs incubated onto the electrode was increased and a higher ECL signal was obtained. The constructed biosensor exhibited a sensitive response towards Con A and a low detection limit of 0.16 pg/mL was obtained. Such a construction strategy provides a new approach for applying GQDs to establish a solid-state ECL biosensor.

以石墨烯量子点（GQDs）为发光体，构建了用于检测伴刀豆球蛋白A（Con A）的固态电化学发光（ECL）生物传感器，通过将它们之间的相互作用将其装载在聚酰胺（PAMAM）功能化的CeO ₂ @Ag NP上。PAMAM的NH ₂和GQD的COOH。电沉积金层用作固定葡萄糖氧化酶（GOx）的基质。GOx充当识别元素，通过碳水化合物和Con A之间的特异性相互作用进一步捕获ConA。随着Con A浓度的增加，GOx-CeO ₂的量增加培养在电极上的@ Ag-GQD增加，并且获得了更高的ECL信号。所构建的生物传感器对Con A表现出灵敏的响应，并获得了0.16 pg / mL的低检测限。这种构建策略为应用GQD建立固态ECL生物传感器提供了一种新方法。