A novel capillary electrophoresis with laser-induced fluorescence detection method has been developed for the analysis of aldehyde metabolism biomarkers for oxidative stress in exhaled breath condensate (EBC), and fluorescein 5-thiosemicarbazide was used as a derivatization reagent. In a simple capillary zone electrophoresis mode, ten low molecular weight aldehydes (LMWAs) could be well separated within 30 min. The reaction efficiency was doubled by increasing sample solution pH and magnetic stirring, and the LODs of this method reached 0.16–3.4 nM (S/N = 3). Acceptable recoveries (82.1–115%) were obtained for EBC samples, and the RSD data were within 7.9%. This developed method has been applied for the analyses of EBC samples and evaluation of the correlation between smoking and the contents of aldehyde metabolites in EBC. Due to no need of buffer additives and sample preconcentration, this proposed method may provide an appealing alternative for the trace analyses of LMWAs in noninvasive biofluids.

中文翻译：

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毛细管电泳-激光诱导荧光检测灵敏测定呼出气冷凝物中的醛代谢物

开发了一种新型的毛细管电泳激光诱导荧光检测方法，用于分析呼出气冷凝液（EBC）中氧化应激的醛代谢生物标志物，并使用了荧光素5-硫代氨基脲作为衍生试剂。在简单的毛细管区带电泳模式下，可以在30分钟内很好地分离十种低分子量醛（LMWA）。通过提高样品溶液的pH值和磁力搅拌，反应效率提高了一倍，该方法的检测限达到0.16–3.4 nM（S / N = 3）。EBC样品的回收率可接受（82.1–115％），RSD数据在7.9％以内。这种发达的方法已用于分析EBC样品以及评估吸烟与EBC中醛代谢物含量之间的相关性。由于不需要缓冲液添加剂和样品预浓缩，因此该方法可为非侵入性生物流体中的LMWA痕量分析提供有吸引力的替代方法。