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Vessel‐on‐a‐chip with Hydrogel‐based Microfluidics
Small ( IF 13.3 ) Pub Date : 2018-10-11 , DOI: 10.1002/smll.201802368
Jing Nie 1, 2 , Qing Gao 1, 2 , Yidong Wang 3 , Jiahui Zeng 1, 2 , Haiming Zhao 1, 2 , Yuan Sun 1, 2 , Jian Shen 3 , Hamed Ramezani 1, 2 , Zhenliang Fu 1, 2 , Zhenjie Liu 4 , Meixiang Xiang 3 , Jianzhong Fu 1, 2 , Peng Zhao 1, 2 , Wei Chen 5 , Yong He 1, 2
Affiliation  

Hydrogel structures equipped with internal microchannels offer more in vivo‐relevant models for construction of tissues and organs in vitro. However, currently used microfabrication methods of constructing microfluidic devices are not suitable for the handling of hydrogel. This study presents a novel method of fabricating hydrogel‐based microfluidic chips by combining the casting and bonding processes. A twice cross‐linking strategy is designed to obtain a bonding interface that has the same strength with the hydrogel bulk, which can be applied to arbitrary combinations of hydrogels. It is convenient to achieve the construction of hydrogel structures with channels in branched, spiral, serpentine, and multilayer forms. The experimental results show that the combination of gelatin and gelatin methacrylate (GelMA) owns the best biocompatibility and can promote cell functionalization. Based on these, a vessel‐on‐a‐chip system with vascular function in both physiological and pathological situations is established, providing a promising model for further investigations such as vascularization, vascular inflammation, tissue engineering, and drug development. Taken together, a facile and cytocompatible approach is developed for engineering a user‐defined hydrogel‐based chip that can be potentially useful in developing vascularized tissue or organ models.

中文翻译:

带有基于水凝胶的微流体的单芯片血管

配备内部微通道的水凝胶结构为体内组织和器官的构建提供了更多与体内相关的模型。然而,当前使用的构造微流体装置的微制造方法不适合于水凝胶的处理。这项研究提出了一种通过结合浇铸和粘结工艺来制造基于水凝胶的微流控芯片的新方法。设计了两次交联策略,以获得与水凝胶块具有相同强度的键合界面,该界面可以应用于水凝胶的任意组合。方便地构造具有分支,螺旋,蛇形和多层形式的通道的水凝胶结构。实验结果表明,明胶和甲基丙烯酸明胶(GelMA)的组合具有最佳的生物相容性,并可以促进细胞功能化。在此基础上,建立了在生理和病理情况下均具有血管功能的片上血管系统,为进一步研究,例如血管形成,血管炎症,组织工程和药物开发提供了一个有希望的模型。综上所述,开发了一种简便且可与细胞兼容的方法,用于工程设计用户定义的基于水凝胶的芯片,该芯片可能对开发血管化的组织或器官模型有用。为进一步研究提供了有希望的模型,例如血管形成,血管炎症,组织工程和药物开发。综上所述,开发了一种简便且可与细胞相容的方法,用于工程设计用户定义的基于水凝胶的芯片,该芯片可能对开发血管化的组织或器官模型有用。为进一步研究提供了有希望的模型,例如血管形成,血管炎症,组织工程和药物开发。综上所述,开发了一种简便且可与细胞相容的方法,用于工程设计用户定义的基于水凝胶的芯片,该芯片可能对开发血管化的组织或器官模型有用。
更新日期:2018-10-11
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