Abstract In this study, 111 ready-to-eat anchovy products were collected on the Italian market. The products were molecularly identified through a BLAST analysis of a highly informative cytb fragment amplified by a newly designed primer pair for the genus Engraulis spp. and the mislabelling rate was assessed. In addition, the labels were analysed in the light of the current EU law. Despite only one mislabelling case was observed (mislabelling rate 0.9%), which involved the substitution of the European anchovy (Engraulis encrasicolus) with the low-valuable Peruvian/Chilean anchoveta (Engraulis ringens), the molecular technique developed in this study was proved as suitable tool for detecting species in processed anchovy products. It could be therefore applied to carry out more extensive EU survey aimed at evaluating the mislabelling rate of such products, still poorly covered by a targeted and clear legislation system.

中文翻译：

---

通过对信息丰富的细胞色素 b 基因片段的 BLAST 分析，对意大利市场上销售的即食凤尾鱼产品进行认证

摘要 本研究收集了意大利市场上的 111 种即食凤尾鱼产品。通过对由新设计的 Engraulis 属的引物对扩增的高信息 cytb 片段进行 BLAST 分析，对产物进行分子鉴定。并对误贴率进行了评估。此外，根据现行欧盟法律对标签进行了分析。尽管仅观察到一个错误标记案例（错误标记率为 0.9%），其中涉及用低价值的秘鲁/智利凤尾鱼 (Engraulis ringens) 替代欧洲凤尾鱼 (Engraulis encrasicolus)，但本研究中开发的分子技术被证明为用于检测加工鳀鱼产品中种类的合适工具。