Laser ablation (LA) coupled with inductively coupled plasma mass spectrometry (ICP-MS) is a versatile tool for direct trace elemental and isotopic analysis of solids. The development of new strategies for quantitative elemental mapping of biological tissues is one of the growing research areas in LA-ICP-MS. On the other hand, the latest advances are related to obtaining not only the elemental distribution of heteroatoms but also molecular information. In this vein, mapping of specific proteins in biological tissues can be done with LA-ICP-MS by use of metal-labelled immunoprobes. However, although LA-ICP-MS is, in principle, a quantitative technique, critical requirements should be met for absolute quantification of protein distribution. In this review, progress based on the use of metal-labelled antibodies for LA-ICP-MS mapping of specific proteins is reported. Critical requirements to obtain absolute quantitative mapping of specific proteins by LA-ICP-MS are highlighted. Additionally, illustrative examples of the advances made so far with LA-ICP-MS are provided.

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激光烧蚀（LA）与电感耦合等离子体质谱仪（ICP-MS）结合使用，是直接对固体进行痕量元素和同位素分析的通用工具。在生物组织中进行定量元素标测的新策略的开发是LA-ICP-MS不断发展的研究领域之一。另一方面，最新进展不仅与获得杂原子的元素分布有关，而且与分子信息有关。在这种情况下，可以使用LA-ICP-MS通过使用金属标记的免疫探针对生物组织中的特定蛋白质进行定位。但是，尽管LA-ICP-MS原则上是一种定量技术，但对于蛋白质分布的绝对定量，应满足关键要求。在这篇评论中，据报道，基于金属标记抗体的LA-ICP-MS定位特定蛋白质的研究进展。突出显示了通过LA-ICP-MS获得特定蛋白质的绝对定量作图的关键要求。此外，还提供了迄今为止用LA-ICP-MS取得的进步的说明性示例。

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