An integrated production of methyl ester and ɛ-polylysine from Chlamydomonas sp. was studied using biorefinery approach. The harvesting efficiency of Chlamydomonas sp. was increased up to 92% by treatment with a flocculant FeCl₃ at 100 mg/L for 30 min. The DMC (dimethyl carbonate) mediated enzyme catalyzed in-situ transesterification of Chlamydomonas sp. yielded the maximum methyl ester of 92% under optimized conditions. The valued-added product ɛ-polylysine was produced from hydrolysate obtained from the spent biomass of Chlamydomonas sp. using Streptomyces sp. The key components of sugar and MgSO₄ used for ɛ-polysine production were optimized whereby the maximum ɛ-polylysine production was achieved at 50 g/L sugar and 0.3 g/L MgSO₄. The ɛ-polylysine production was further enhanced by supplementation of important amino acids (lysine and aspartate) and TCA cycle intermediates (citric acid and α-ketoglutaric acid). The maximum ɛ-polylysine production of 2.24 g/L was found with 4 mM citric acid supplementation after 110 h.

由衣藻属（Chlamydomonas sp。）综合生产甲酯和α-聚赖氨酸。使用生物精炼方法进行了研究。衣藻的收获效率。通过用絮凝剂FeCl ₃以100 mg / L的浓度处理30分钟，其含量可增加至92％。DMC（碳酸二甲酯）介导的酶催化衣原体属的原位酯交换反应。在优化条件下产生的最大甲酯含量为92％。有价值的产品ɛ-聚赖氨酸是从衣藻属的废生物质中获得的水解产物生产的。使用链霉菌属。糖和MgSO ₄的关键成分优化了用于α-聚赖氨酸生产的糖，从而在50 g / L糖和0.3 g / L MgSO _4的情况下实现了最大的γ-聚赖氨酸生产。通过补充重要的氨基酸（赖氨酸和天冬氨酸）和TCA循环中间体（柠檬酸和α-酮戊二酸），可进一步提高β-聚赖氨酸的产量。110 h后补充4 mM柠檬酸时，最大α-聚赖氨酸产量为2.24 g / L。