Ionizing radiation-induced skin injury is a common and severe side effect of radiotherapy suffered by cancer patients. Although the therapy using stem cells has been demonstrated to be effective, fully grasping their role in the repair of radiation-induced skin damage remains challenging owing to the lack of highly reliable cell trackers. Herein, we report the design and synthesis of a highly near-infrared emissive organic nanodots with aggregation-induced emission (AIE) characteristic, which give excellent performance in seeing the fate and regenerative mechanism of adipose-derived stem cells (ADSCs) in treatment of radiation-induced skin injury. The resultant AIE dots show a rather high quantum yield of 33% in aqueous media, prominent retention ability in ADSCs without leakage, good biocompatibility during the ADSC differentiation and proliferation as well as excellent injury relief capability on radiation-induced endothelial cells injury. In vivo studies reveal that the AIE dots are capable of serving as an effective fluorescent cell tracker to precisely trace the behavior of the transplanted ADSCs in radiation-induced skin injury-bearing mice and help to understand the ADSCs therapeutic mechanism for at least one month. This study will provide new materials and insights into the stem cell therapy of radiation-induced injury.

中文翻译：

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了解使用高度近红外发射AIE点治疗电离辐射引起的损伤的干细胞的命运和机制。

电离辐射引起的皮肤损伤是癌症患者遭受放射治疗的常见且严重的副作用。尽管使用干细胞的疗法已被证明是有效的，但由于缺乏高度可靠的细胞追踪剂，充分掌握其在修复辐射诱发的皮肤损伤中的作用仍然具有挑战性。在此，我们报告具有聚集诱导发射（AIE）特性的高度近红外发射有机纳米点的设计和合成，这些纳米点在观察脂肪来源干细胞（ADSCs）的命运和再生机制方面具有出色的表现。辐射引起的皮肤损伤。所得的AIE点在水性介质中显示出相当高的量子产率，为33％，在ADSC中具有显着的保留能力，而不会泄漏，在ADSC分化和增殖过程中具有良好的生物相容性，并且对放射线诱导的内皮细胞损伤具有出色的损伤缓解能力。体内研究表明，AIE点能够用作有效的荧光细胞追踪剂，以精确追踪移植的ADSC在辐射诱发的皮肤损伤小鼠中的行为，并有助于了解ADSC至少一个月的治疗机制。这项研究将为放射损伤的干细胞疗法提供新的材料和见识。体内研究表明，AIE点能够充当有效的荧光细胞追踪剂，以精确追踪在辐射诱发的皮肤损伤小鼠中移植的ADSC的行为，并有助于了解ADSC至少一个月的治疗机制。这项研究将为放射损伤的干细胞疗法提供新的材料和见识。体内研究表明，AIE点能够用作有效的荧光细胞追踪剂，以精确追踪移植的ADSC在辐射诱发的皮肤损伤小鼠中的行为，并有助于了解ADSC至少一个月的治疗机制。这项研究将为放射损伤的干细胞疗法提供新的材料和见识。