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Nanoporous silica nanoparticles functionalized with a fluorescent turn-on spirorhodamineamide as pH indicators†
Photochemical & Photobiological Sciences ( IF 3.1 ) Pub Date : 2018-10-11 00:00:00 , DOI: 10.1039/c8pp00133b
M. Di Paolo 1, 2, 3, 4, 5 , M. J. Roberti 1, 2, 3, 4 , A. V. Bordoni 4, 6, 7, 8 , P. F. Aramendía 4, 5, 9, 10, 11 , A. Wolosiuk 4, 5, 6, 9, 10 , M. L. Bossi 1, 2, 3, 4, 5
Affiliation  

We prepared water soluble, biocompatible fluorescent turn-on pH nanosensors and characterized their behavior as a function of changes in pH. The response relies on a halochromic reaction of a spirorhodamineamide derived from the bright and highly chemically and photo-stable rhodamine 6G, encapsulated in core/nanoporous shell silica nanoparticles. The fluorescent sensors displayed a fast response in the pH range of intracellular compartments. The encapsulation conferred solubility in aqueous environments and biocompatibility. We assessed the two main properties of the sensor, namely the useful pH range and the kinetics of the response, and compared them to those of the free probe. We found that such properties are strongly dependent on the functionalization and position in the silica matrix relative to the core/shell structure. Finally, we demonstrated the cellular uptake of the nanosensors, and their localization in lysosomes of living cells, by fluorescence confocal microscopy.

中文翻译:

纳米多孔二氧化硅纳米粒子,其功能为使用荧光开启螺螺胺作为pH指示剂

我们准备了水溶性,生物相容性的荧光开启pH纳米传感器,并根据pH的变化表征了其行为。该响应依赖于螺环罗丹明酰胺的色变反应,该螺罗丹明酰胺是由包裹在核/纳米孔壳二氧化硅纳米颗粒中的明亮,高度化学和光稳定的若丹明6G衍生的。荧光传感器在细胞内区室的pH范围内显示出快速响应。包封赋予在水性环境中的溶解度和生物相容性。我们评估了传感器的两个主要特性,即有用的pH范围和响应的动力学,并将其与游离探针的特性进行了比较。我们发现,这种性质在很大程度上取决于官能度和相对于核/壳结构在二氧化硅基质中的位置。最后,
更新日期:2018-10-11
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