Lentivirus and other retroviral vectors are useful tools to engineer chimeric antigen receptor (CAR) T cells which are designed to target tumor cells in the growing field of gene therapy. Several efforts have been made to improve vector packaging systems including plasmid systems and envelopes and develop stable packaging cell lines (PCLs) in order to improve the productivity and safety by eliminating cytotoxicity of HIV-1 genes and unnecessary homologous genomic regions. In this paper, we explored stable PCLs developed to date for the production of both lentiviral and retroviral vectors and addressed key features of the viral vector production systems and how the features can be further utilized and modified to meet the growing demand in clinical trials.

慢病毒和其他逆转录病毒载体是工程化嵌合抗原受体（CAR）T细胞的有用工具，这些嵌合抗原受体T细胞被设计为在基因治疗的不断发展的领域中靶向肿瘤细胞。为了消除包括HIV-1基因和不必要的同源基因组区域的细胞毒性，为了提高生产率和安全性，已经做出了一些努力来改善包括质粒系统和包膜的载体包装系统，并开发稳定的包装细胞系（PCL）。在本文中，我们探讨了迄今为止为生产慢病毒和逆转录病毒载体而开发的稳定PCL，并论述了病毒载体生产系统的关键特征以及如何进一步利用和修饰这些特征以满足临床试验中不断增长的需求。