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Assessment of selenium bioaccumulation in lactic acid bacteria
Journal of Dairy Science ( IF 3.5 ) Pub Date : 2018-10-11 , DOI: 10.3168/jds.2018-14852
Ana Paula Mörschbächer , Anja Dullius , Carlos Henrique Dullius , Cassiano Ricardo Bandt , Daniel Kuhn , Débora Tairini Brietzke , Fernando José Malmann Kuffel , Henrique Pretto Etgeton , Taciélen Altmayer , Tamara Engelmann Gonçalves , Ytan Andreine Schweizer , Eliézer Quadro Oreste , Anderson Schwingel Ribeiro , Daniel Neutzling Lehn , Claucia Fernanda Volken de Souza , Lucélia Hoehne

Selenium is an essential micronutrient for living beings, as it helps to maintain the normal physiological functions of the organism. The numerous discoveries involving the importance of this element to the health of human beings have fostered interest in research to develop enriched and functional foods. The present study evaluated the potential for bacterial strains of Enterococcus faecalis (CH121 and CH124), Lactobacillus parabuchneri (ML4), Lactobacillus paracasei (ML13, ML33, CH135, and CH139), and Lactobacillus plantarum (CH131) to bioaccumulate Se in their biomass by adding different concentrations of sodium selenite (30 to 200 mg/L) to the culture medium. Quantification of Se with UV and visible molecular absorption spectroscopy showed that the investigated bacteria were able to bioaccumulate this micromineral into their biomass. Two of the L. paracasei strains (ML13 and CH135) bioaccumulated the highest Se concentrations (38.1 ± 1.7 mg/g and 40.7 ± 1.1 mg/g, respectively) after culture in the presence of 150 mg/L of Se. This bioaccumulation potential has applications in the development of dairy products and may be an alternative Se source in the diets of humans and other animals.



中文翻译:

乳酸菌中硒生物富集的评估

是生物必需的微量营养素,因为它有助于维持有机体的正常生理功能。涉及该元素对人类健康重要性的众多发现,激发了人们对开发富含功能食品的研究兴趣。本研究评估了肠球菌(CH121和CH124),布氏乳杆菌( ML4),副干酪乳杆菌(ML13,ML33,CH135和CH139)和植物乳杆菌的细菌菌株的潜力(CH131)可通过向培养基中添加不同浓度的亚硒酸钠(30至200 mg / L)在生物质中生物富集硒。用紫外线和可见分子吸收光谱对硒进行定量分析表明,所研究的细菌能够将这种微量矿物质生物积累到其生物质中。在150 mg / L Se的条件下培养后,副干酪乳杆菌中的两个菌株(ML13和CH135)生物富集了最高的Se浓度(分别为38.1±1.7 mg / g和40.7±1.1 mg / g)。这种生物蓄积潜力可用于乳制品的开发中,并且可以作为人类和其他动物饮食中硒的替代来源。

更新日期:2018-10-11
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